miR-361-5p对激素非依赖前列腺癌细胞PC3增殖、侵袭的影响  

Effects of miR-361-5p on proliferation and invasion in androgen-independent human prostate cancer cell line PC3

作　　者：刘大闯[1,3] 陶陶[1] 许斌[1] 陈恕求[1] 刘春辉[1] 张磊[1] 卢凯[1] 陈明[1] 韩从辉[2,3] 

机构地区：[1]东南大学附属中大医院泌尿外科,南京210009 [2]东南大学附属徐州市中心医院泌尿外科 [3]徐州医学院附属徐州临床学院泌尿外科

出　　处：《中国男科学杂志》2014年第8期3-8,共6页Chinese Journal of Andrology

基　　金：本课题受国家自然基金（81370849、81300472、81272557、81202034）、临床医学科技专项-新型临床诊疗技术攻关（BL2013032）、教育部博士点基金（20120092120071）、江苏省自然科学基金（BK2012336、BK2012647）、南京市科技发展项目（201201053）、东南大学新教师基本科研项目（3290002402）、江苏省普通高校研究生科研创新计划项目（CXZZ130133）和中央高校基本科研业务费专项资金资助资助

摘　　要：目的：探讨mirR-361-5p在激素非依赖性前列腺癌细胞PC3中的表达和作用。方法采用qRT-PCR检测转染后的PC3中miRNA-361-5p的表达情况，用CCK-8和克隆形成实验检测转染后的PC3细胞增殖能力，流式细胞仪检测细胞周期和细胞凋亡的变化，Transwell实验检测细胞侵袭能力。结果 qRT-PCR检测结果显示PC3中miR-361-5p含量低，脂质体法有较高的转染效率。CCK-8与克隆形成实验表明在PC3中升高miR-361-5p能够抑制前列腺癌细胞的活性和增殖能力。流式细胞检测发现miR-361-5p可增加细胞阻滞在G0/G1期和凋亡的比例。Transwell实验中，转染miR-361-5p后细胞通过小室的数量明显减少。结论 miR-361-5p能够抑制PC3的增殖与侵袭能力，从而发挥抑癌的作用。Objective To investigate the effects of mirR-361-5p on bilogical behaviors of androgen-independent human prostate cancer cellsPC3. Methods The expression of miR-361-5p in PC3 cells after transfection was detected by qRT-PCR. Cell viability and proliferation of PC3 cells trasfected with miR-361-5p were measured by Cell Counting Kit-8（CCK-8） assay and colony formation assay. Cell cycle and apoptosis of PC3 cells transfected with miR-361-5p were analyzed by flow cytometry. The invasion ability of cell was evaluated by Transwell assay. Results The results of qRT-PCR showed that the expression of miR-361-5p in PC3 cells was low, indicating transfection efficiency was higher using liposome method. Overexpression of miR-361-5p significantly inhibited the proliferation of PC3 cells. Cell cycle analysis of PC3 cells transfected with miR-361-5p showed that cell number in G0/G1 phase was high and cell apoptosis number was low. MiR-361-5p significantly inhibited the number of cells migrated through Transwell chambers membrane. Conclusion These findings suggest that miR-361-5p may be a tumor suppressor miR in PC3 and it can inhibit the proliferation and invasion ability of androgen-independent human prostate cancer cells.

关 键 词：前列腺肿瘤 细胞增殖 肿瘤侵袭力 

分 类 号：R737.25[医药卫生—肿瘤]