分子印迹固相萃取-液相色谱法测定食品中硝基呋喃类药物残留  

Determination of Nitrofuran Antibiotic Residues in Foods by Molecularly Imprinted Solid Phase Extraction-Liquid Chromatography

作　　者：高文惠[1] 王姣姣[1] 贾英民[1] 

机构地区：[1]河北科技大学生物科学与工程学院,石家庄050018

出　　处：《中国食品学报》2014年第9期183-189,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家科技支撑计划项目(2013BAD10B03);河北省科技支撑计划项目(14227504D;14236602D-13)

摘　　要：分别以氨基脲(SEM)和1-氨基乙内酰脲(AHD)为模板分子,α-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,采用本体聚合方式合成分子印迹聚合物(MIP),建立了分子印迹固相萃取-液相色谱法检测食品中硝基呋喃类药物残留的方法。将MIP制成固相萃取小柱用于样品的前处理过程,并用反相高效液相色谱测定样品中的硝基呋喃类抗生素药物残留。结果表明,SEM和AHD的线性范围分别为1~200μg/g和0.5~200μg/g,线性相关系数分别为0.9991和0.9987。在1.0,5.0,50.0μg/g 3个添加水平下,这两种代谢物的平均回收率在81.0%~85.5%之间,相对标准偏差(RSD)在1.98%~5.01%(n=6)。该方法简便快速、准确可行。Two molecularly imprinted polymers （MIPs） were prepared by the method of bulk polymerization using semiearbazide （SEM） and 1-aminohy-dantoin （AHD） as the template molecules, using methacbrylic acid（MAA） and ethylene glycol dimethacrylate （EDMA） as functional monomer and cross-linker, respectively. A new method of molecularly imprinted solid phase extraction-liquid chromatography was established for determination of nitrofuran antibiotic residues in foods. The two MIPs were used for the preparation of solid-phase extraction column and sample pre-treatment. Nitrofuran antibiotic residues in the foods derived from animals were tested by reverse phase high performance liquid chromatogra- phy. The results showed that the linear ranges of SEM and AHD were 1-200 μg/mL and 0.5 μg/mL^200 μg/mL, respec- tively, the linear correlation coefficients were 0.9991 and 0.9987, respectively. Under three added concentrations of 1.0μg/g, 5.0 μg/g and 50.0μg/g, the average recoveries for the two kinds of metabolites were in the range of 81.0% and 85.5%, and the relative standard deviations （RSD） were in the range of 1.98% and 5.01%（n=6）. The method was simple and fast,accurate and feasible.

关 键 词：氨基脲 1-氨基乙内酰脲 分子印迹聚合物 固相萃取 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS207.53[理学—化学]