积雪草酸抑制小鼠主动脉缩窄术后心肌肥厚形成  

Protective effects of asiatic acid against postoperative cardiac hypertrophy induced by transverse aortic constriction in mice

作　　者：司林杰[1] 许晶[1] 易晨龙 徐晓晗[1] 张杨杨[1] 张郁青[2] 王晓伟[1] 

机构地区：[1]南京医科大学第一附属医院胸心外科,江苏南京210029 [2]南京医科大学附属江宁医院心血管内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2014年第9期1168-1172,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅自然科学基金(12KJB320003);江苏省中医药局科技基(lz13217);南京市科技局科技发展基金(201303036)

摘　　要：目的:观察积雪草酸(asiatic acid,AA)对小鼠主动脉弓缩窄术后心肌肥厚的保护作用并探讨其可能的机制。方法:采用主动脉弓缩窄(transverse aortic constriction,TAC)法建立小鼠心肌肥厚模型。实验分为5组:假手术组(Sham组)、手术组(TAC组)、TAC+AA1[25 mg/(kg·d)]组、TAC+AA2[50 mg/(kg·d)组、TAC+AA3[100 mg/(kg·d)]组。TAC术后2周,心脏超声检测心功能,Western blot法检测心肌组织中转化生长因子(transforming growth factor,TGF)-β1、p-p38、p38、p-ERK1/2以及ERK1/2蛋白的表达,real-time PCR法检测心肌组织中ANP和TGF-β1基因的表达。结果:AA可显著抑制TAC诱导的心肌肥厚,改善心功能,降低TGF-β1基因和蛋白表达,并抑制p38和ERK1/2磷酸化。结论:AA治疗可抑制心肌肥厚,其机制可能与AA抑制TGF-β1-p38/ERK1/2信号通路有关。Objective：To determine the protective effects and molecular mechanisms of asiatic acid （AA） on postoperative cardiac hypertrophy induced by transverse aortic constriction in mice. Methods：Transverse aortic constriction（TAC） was performed to establish the mice model of myocardial hypertrophy. C57BL/6 mice were divided into five groups：the Sham group,the TAC group,the TAC＋AA1 [25 mg/（kg·day）] group;the TAC＋AA2 [50 mg/（kg·day）1 group and the TAC＋AA3 [ 100 mg/（kg·day）] group. After 2 weeks of TAC, echocardiography was performed to measure the hypertrophic criteria of the hearts. The protein expressions of TGF-β1, p-p38,p38,p-ERKl/2 and ERK1/2 were detected by Western blot. The mRNA expressions of ANP and TGF-β1 were detected by real-time PCR. Results：AA significantly inhibited cardiac hypertrophy induced by TAC,improved cardiac function,reduced the mRNA and protein expression of TGF-β1,as well as the phosphorylation of p38 and ERK1/2. Conclusion：AA attenuates TAC- induced cardiac hypertrophy. The protective mechanisms of AA may through inhibiting TGF-β1-p38/ERK1/2 signaling pathway.

关 键 词：积雪草酸 心肌肥厚 转化生长因子Β1 丝裂原激活蛋白激酶 

分 类 号：R541[医药卫生—心血管疾病]