鼻咽癌患者外周血T淋巴细胞及癌组织IL-21蛋白表达变化及意义  

Clinical significance of changes in levels of peripheral blood T lymphocyte and IL-21 protein expression

作　　者：王艳[1,2] 赵颖海[1] 王晓玫 成志强 刘汉勇 吴民华[1] 胡锦涛 赵利容[4] 陈小毅 

机构地区：[1]广东医学院,广东湛江524023 [2]深圳市龙华新区人民医院 [3]深圳市人民医院 [4]四川省宜宾卫生学校 [5]无

出　　处：《山东医药》2014年第18期4-7,共4页Shandong Medical Journal

基　　金：国家重点基础研究发展计划(2011CB504800)

摘　　要：目的观察鼻咽癌患者外周血T淋巴细胞及癌组织IL-21蛋白的表达变化,并探讨其意义。方法采用流式细胞术检测29例鼻咽癌放疗前患者(A组)、34例鼻咽癌放疗后患者(B组)、7例血浆EBV-VCA-IgA阳性鼻咽癌高危者(C组,非鼻咽癌患者)和28例血浆EBV-VCA-IgA阴性的健康体检者(对照组)外周血CD+3、CD+4、CD+8、CD+25T细胞亚群;采用免疫组化法检测52例鼻咽癌、15例鼻咽黏膜慢性炎症组织中的IL-21蛋白,分析IL-21蛋白表达与鼻咽癌临床病理参数的关系。结果 A组CD+3、CD+4细胞百分比及CD+4/CD+8值低于另三组,CD+8细胞百分比高于另三组(P均<0.05);A、B组CD+25细胞百分比均高于C组及对照组(P均<0.05);鼻咽癌组织中IL-21阳性表达率高于鼻咽黏膜慢性炎症组织(P<0.05);Ⅰ+Ⅱ期鼻咽癌患者肿瘤组织中IL-21阳性表达率高于Ⅲ+Ⅳ期者,高分化腺癌中IL-21表达阳性率高于低分化腺癌者(P均<0.05)。结论鼻咽癌患者癌组织中IL-21蛋白表达上调,外周血CD+8细胞百分比提高,二者均与鼻咽癌的发病有关。Objective To investigate the clinical significance of changes in levels of IL-21 protein and peripheral blood T lymphocyte in carcinoma tissues of patients with nasopharyngeal carcinoma （ NPC） and to initially dicuss the rela-tionship between latent infection of EBV and cellular immunity of nasopharyngeal microenvironment and peripheral blood . Methods The peripheral blood CD 3^＋, CD4^＋, CD8^＋, CD2^＋5 T lymphocyte subsets were detected by flow cytometry in 29 cases of NPC patients without radiotherapy （ group A ） , 34 cases of NPC patients with radiotherapy （ group B ） , 7 cases of EBV-VCA-IgA positive people （ group C） and 28 cases of EBV-VCA-IgA negative people （ control group ） .Immunohisto-chemistry was adopted to detect IL-21 protein expression in 52 cases of NPC tissues and 15 cases of chronic nasopharyngitis tissues and the relationship between IL-21 protein expression and the clinicopathological parameters was analyzed .Results The percentages of peripheral blood CD 3^＋, CD4^＋T lymphocytes, the ratio of CD4^＋/CD8^＋, and the percentage of CD8＋in the group A were higher than the other three groups （all P〈0.05）.The percentage of CD2^＋5 T lymphocytes in the group A and B was higher than that of the group C and the control group （all P〈0.05）.The positive expression rate of IL-21 in the NPC tissues was higer than that of chronic rhinitis mucosa （P〈0.05）.The positive expression rate of IL-21 in the I ＋II stage NPC tissues of patients was higher than that in stage III ＋IV, and the positive expression rate of IL-21 of high-differ-entiated adenocarcinoma was also higher than that of low-differentiated adenocarcinoma （all P〈0.05）.Conclusion The expression of IL-21 protein in the NPC tissues of NPC patients is up-regulated , and peripheral blood T cell percentage increases, both of which are associated with the pathogenesis of nasopharyngeal carcinoma .

关 键 词：鼻咽肿瘤 鼻咽癌 T细胞亚群 细胞免疫 白细胞介素-21 

分 类 号：R739.63[医药卫生—肿瘤]