线栓长度对颈内动脉穿刺法建立大鼠蛛网膜下腔出血模型的影响  

INFLUENCES OF THE FILAMENT LENGTH ON THE RAT SUBARACHNOID HEMORRHAGE MODEL BY INTERNAL CAROTID ARTERY PUNCTURING

作　　者：李建华[1] 孙娟[1,2] 张艳[2] 杨磊[2] 闫军浩[2] 

机构地区：[1]青海大学医学院,青海西宁810001 [2]北京大学医学部,北京100191

出　　处：《青海医学院学报》2014年第3期191-195,共5页Journal of Qinghai Medical College

摘　　要：目的在大鼠蛛网膜下腔出血（subarachnoid hemorrhage,SAH）模型造模中,摸索选择微创且重复性好的颈内动脉穿刺线栓长度。方法行颈内动脉穿刺时,将大鼠线栓长度随机分为≤17 mm组、18-20 mm组、20-22 mm组、22-24 mm组和≥24 mm组。在SAH后24 h,通过Garcia神经功能评分、死亡率及出血严重程度等指标判定SAH模型的成功率。结果线栓长度在20-22 mm时,SAH动物成功率最高。结论线栓长度在20-22 mm时,大鼠SAH模型稳定可靠,重复性好,适合进行临床前循环动脉瘤性SAH后早期脑损伤的病理生理研究。Objective To establish a minimally- invasive and repetitive subarachnoid hemorrhage（ SAH） rat model through exploring the filament length. Methods The rats were divided into following groups based on the filament length： ≤17 mm group（ n = 10）,18- 19 mm group（ n = 10）,20- 22 mm group（ n = 10）,22- 24 mm group（ n =10）,and ≥24 mm group（ n =10）. After SAH,neurological scoring,mortality,gross specimens and bleeding severity were evaluated. Results Animal models of SAH were established by endovascular puncture. In the filament of 20- 22 mm,the mortality was lower and the model success rates were better than those of other groups. Conclusion SAH model established with 20- 22 mm filament is stable and reliable,with high repeatability,suitable for studying the early brain injury in clinical anterior circulation aneurysm SAH.

关 键 词：线栓 蛛网膜下腔出血 动物模型 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]