胶东地区健康禽源大肠埃希菌耐药性与整合子-基因盒携带分析  

Analysis of Drug Resistance and Integron-Gene Cassette Carring in Escherichia coli Isolated from Health Poultry in Jiaodong Region

作　　者：刘红玉[1,2] 王君玮[1] 王娟[1] 赵思俊[1] 黄秀梅[1] 李玉清[1] 曲志娜[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]东北农业大学,黑龙江哈尔滨150030

出　　处：《动物医学进展》2014年第10期66-70,共5页Progress In Veterinary Medicine

基　　金："十二五"科技基础性工作专项项目(2012FY111000)

摘　　要：为了解健康禽源大肠埃希菌的耐药性及其Ⅰ型和Ⅱ型整合子携带情况和流行特征,探讨整合子与大肠埃希菌耐药表型的相关性,分析整合子在细菌多重耐药中的作用,采用MIC法和PCR方法分别检测249株健康禽源大肠埃希菌的耐药性和Ⅰ型、Ⅱ型整合酶基因及可变区。结果显示,12种药物耐药率均超过56.63%,多重耐药率高达98.39%;245株多重耐药菌株均含有Ⅰ型整合子,但含有Ⅱ型整合子的大肠埃希菌仅113株,Ⅰ型和Ⅱ型整合子的检出率分别为100%和46.12%;63.75%的Ⅰ型整合子阳性菌株扩增出5类大小不同的基因盒,大小为1 000bp^23 00bp,18.6%的Ⅱ型整合子阳性菌株扩增出2 200bp的基因盒;58.82%的菌株携带2种或2种以上的基因盒,基因盒以编码甲氧苄啶类和氨基糖苷类药物耐药性的dfrA和aadA基因家族为主,其中aadA22为优势基因盒,其次是dfrA17+aadA5基因盒。说明胶东地区禽源大肠埃希菌多重耐药现象严重;Ⅰ型整合子比Ⅱ型整合子更流行;Ⅰ型和Ⅱ型整合子参与多重耐药,但细菌携带的整合子-基因盒与其耐药表型无对应关系。In order to understand the drug resistance of healthy poultry E.coli and its Ⅰ and Ⅱ integron carried and popular features,discuss the correlation of integron and E.coli drug-resistant phenotype,ana-lyze of the role of integron in multiple drug-resistant bacteria,MIC method and polymerase chain reaction （PCR）were used to determine of antimicrobial drug resistance andⅠ,Ⅱ integrase genes and variable area of 249 healthy poultry E.coli respectively,and the enzyme digestion,sequencing analysis of the resistance gene cassettes carried by integron variable area were made.The results showed that the resistant rate of 12 kinds of drugs was more than 56.63%,multiple resistant rate was as high as 98.39%;245 multiple drug-resistant strains all contained Ⅰ integron,but only 113 strains E.coli contained Ⅱ integron,the detection rates ofⅠ and Ⅱ integron were 100% and 46.12%,respectively ;63.75% ofⅠ integron positive strains amplified out 5 kinds of different size gene cassettes,the size was 1 000-2 300 bp,18.6% ofⅡ integron positive strains amplified out 2 200 bp gene cassette;58.82% of strains carried two or more than two gene cassettes,gene cassettes were priority to encode methoxyl benzyl organism and aminoglycoside drug resist-ant dfrA and aadA gene families,among them aad A22 as dominant gene cassette,followed by dfrA17 ＋aadA5 gene cassettes.The results indicate that the multiple drug-resistance of poultry E.coli from Jia-odong region is serious;Ⅰ integron is more popular than Ⅱ integron,Ⅰ and Ⅱ integron both participate in multiple drug resistance,but integron-gene cassette carried by the bacteria has no corresponding relation with its drug-resistant phenotype.

关 键 词：禽 大肠埃希菌 多重耐药 整合子-基因盒 

分 类 号：Q812.65[生物学—生物工程]