京海黄鸡MyoG基因密码子特性分析  

Characterization of Codon Usage of MyoG Gene in Jinghai Yellow Chicken

作　　者：张涛[1,2] 张跟喜[1,2] 韩昆鹏[1,2] 王金玉[1,2] 樊庆灿[1,2] 李婷婷[1,2] 段炼[1,2] 王永娟 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [3]江苏京海集团,江苏南通226103

出　　处：《华北农学报》2014年第4期71-79,共9页Acta Agriculturae Boreali-Sinica

基　　金：江苏省高校自然科学研究项目(12KJB230003);国家肉鸡产业技术体系项目(nycytx-42-G1-05);江苏高校优势学科建设工程项目;江苏省动物遗传繁育与分子设计重点实验室项目

摘　　要：为了解鸡MyoG基因的密码子特性,以便于为MyoG基因的表达选择合适的外源表达系统,使用在线工具CUSP和CHIPS以及codonW软件对鸡MyoG基因进行分析,并与鸡基因、模式动物基因组和其他动物MyoG基因进行比较。结果表明,MyoG基因偏好于G/C结尾的密码子,CDS序列中,GC含量大于AT含量。MyoG基因与鸡其他31个基因密码子偏好性相似,均偏好于G/C结尾的密码子。与其他物种基因组密码子偏好性比较结果显示,密码子偏好性与小鼠差异最小,说明小鼠可以作为MyoG基因外源表达的宿主。与其他动物MyoG基因密码子偏好性比较显示,MyoG基因在包括京海黄鸡在内的32个物种中均有较高表达,几乎所有MyoG基因编码区对GC有较强的偏好性。基于RSCU值的聚类和基于CDS区系统发育树结果显示,亲缘关系近的物种之间倾向于拥有相似的密码子偏好性。In order to understand the codon characteristics of MyoG gene in chicken and select the appropriate exogenous expression system , online tools CUSP , CHIPS and codonW software were used to analyze MyoG gene . MyoG gene was compared to other genes of chicken ,model animal genomes and MyoG gene of other species .Result showed that MyoG of chicken was bias toward the synonymous codons with G and C at the third codon position .GC content was greater than AT content in CDS region .MyoG gene and other thirty-one genes of chicken shared similar codon bias toward the synonymous codons with G and C at the third codon position .Codon bias comparison to other species showed that differences of codon bias between MyoG gene and Mus musculus was the minimum,indicating that Mus musculus could be used as exogenous gene expression host of MyoG gene.Codon bias comparison to the MyoG gene of other species indicated that MyoG gene expressed high in all the thirty-two species ,including Jinghai yellow chicken .Almost all the thirty-two MyoG genes were bias toward to GC .Result of clustering based on RSCU value and phylogenetic tree based on CDS region showed that species with close genetic relationship tend to have similar codon bias .

关 键 词：京海黄鸡 MYOG 密码子 偏好性 聚类分析 

分 类 号：Q78[生物学—分子生物学]