辣椒花青素生物合成相关基因的表达分析研究  

Research and Analysis of the Expression of Anthocyanin Biosynthesis Related Gene in Pepper

作　　者：李珍[1,2] 刘金兵[2] 刁卫平[2] 王述彬[2] 潘宝贵[2] 戈伟[2] 郭广君[2] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江苏省农业科学院蔬菜研究所,江苏南京210014

出　　处：《华北农学报》2014年第4期87-92,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划项目(2012BAD02B02);现代农业产业技术体系建设专项基金项目(CARS-25);国家高技术研究发展计划"863计划"项目(2012AA100103);江苏省农业科技自主创新基金项目(CX(12)1004)

摘　　要：为了明确辣椒花青素生物合成途径中相关基因的表达调控模式，选取3种不同果实颜色的辣椒品系（紫晶、淡紫、水晶）为试材，提取果实不同发育阶段的RNA并反转录成cDNA，利用qRT-PCR技术分析相关基因（ CHS、CHI、F3H、F3′5′H、DFR、ANS、UFGT、ANP、GST）在不同辣椒材料果实不同发育阶段的表达情况。结果显示：CHS、CHI在3种材料果实发育过程中表达量都很少且无规律可循，F3H在淡紫、紫晶中表达量较高，且在果实发育的第15～20天时出现较大峰值，与之相比在水晶中的表达量可以忽略不计。 F3′5′H、ANP、GST、DFR、UFGT在3种材料中相对表达量随着果实的发育分别呈现同步变化，且F3′5′H、ANP、GST在材料紫晶果实发育的第15天相对表达量出现较大峰值，ANS在3种材料果实发育过程中的相对表达量变化没有一定规律可循。 F3H、F3′5′H、ANP、GST是辣椒花青素代谢途径的关键基因。In order to clarify the expression and regulation patterns of related gene in the process of anthocyanin biosynthesis in pepper ,select three pepper strains which fruit of different color for materials （ Zijing,Danzi,Shui-jing） ,extract RNA of different stages of the fruit and reverse transcribed into cDNA ,then using qRT-PCR technolo-gy to analysis of expression of structure genes （ CHS,CHI,F3H,F3′5′H,DFR,ANS,UFGT,ANP and GST） in pep-per fruit of three kinds of materials in different development stages .The results showed that the expression of CHS, CHI are rarely and disorder in the process of fruit development of three kinds of materials ,the expression of F3H is higher in Danzi and Zijing ,and appear larger peak in the fruit development of 15-20 days,compared with it,the a-mount of expression in Shuijing can be negligible .The relative transcription level of F3′5′H,ANP,GST,DFR and UFGT express synchronous change in three kinds of materials ,and the relative expression of F3′5′H,ANP and GST in the 15 th day of Zijing fruit appeared larger peak ,the relative transcription level of ANS without some rules to fol-low.F3H,F3′5′H,ANP and GST are the key genes to the anthocyanins metabolic pathway in pepper .

关 键 词：辣椒 花青素 生物合成 表达分析 

分 类 号：Q78[生物学—分子生物学]