番鸭细小病毒VP3基因和鸭IL-2基因真核融合表达载体的构建  

Construction of Muscovy duck parvovirus VP3 gene and duck IL-2 eukaryotic fusion expression vector

作　　者：董嘉文[1] 李林林[1] 孙敏华[1] 袁建丰[1] 邝瑞欢[1] 胡奇林[1] 

机构地区：[1]广东省农业科学院动物卫生研究所广东省兽医公共卫生公共实验室,广东广州510640

出　　处：《中国兽医杂志》2014年第8期17-19,共3页Chinese Journal of Veterinary Medicine

基　　金：公益性行业(农业)科研专项(201003012);广东省农业科学院动物卫生研究所所长基金;广东省自然科学基金博士启动项目(S2011040001867)

摘　　要：将番鸭细小病毒(MDPV)主要结构蛋白VP3基因和免疫刺激基序(CpG)依次克隆至质粒pIRES-dIL2上,构建了重组质粒pIRES-dIL2-VP3-CpG。核酸疫苗重组质粒转染BHK-21细胞进行间接免疫荧光试验,可在细胞浆中检测到绿色荧光,表明重组质粒可以成功地在真核细胞内表达。VP3 gene of Muscovy duck parvovirus and CpG motifs were sequenced and cloned into the expression vector pIRES-dIL2.The recombinant plasmid pIRES-dIL2-VP3-CpG was constructed successfully. Then the recombinant plasmid was transfected into BHK-21 cells with LipofectamineTM2000. Green fluorescence of the transfected cells could be observed by indirect immunofluorescence test,demonstrating that expression ofthe recombinant plasmid in eukaryotic cells was successful.

关 键 词：番鸭细小病毒 VP3基因 鸭IL-2基因 CPG基序 真核表达 

分 类 号：S852.65[农业科学—基础兽医学]