检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]四川大学生命科学学院遗传医学研究所,生物资源与生态环境教育部重点实验室,四川610064 [2]重庆市人口和计划生育科学技术研究院,国家卫生计生委出生缺陷与生殖健康重点实验室,重庆400020
出 处:《中国优生与遗传杂志》2014年第9期55-56,81,共3页Chinese Journal of Birth Health & Heredity
摘 要:目的探讨男性不育患者染色体核型异常及无精症因子(AZF)基因缺失与男性不育的关系。方法对2012年5月-2014年5月来本院就诊的(重庆地区)原发性男性不育患者165例,进行外周血G显带核型分析并采用多重PCR对无精症因子区域的15个标签序列位点进行检测。结果165例生精障碍患者中染色体异常共检出5例,1例为男性性反转(46,XX),1例为克氏综合征(47,XXY),1例为47,XY,+mar,1例为46,XY,Y≥18,1例46,XY,in(9),其余均为正常核型,总异常率为3.03%(5/165);AZF基因位点发生微缺失患者共检出25例,总缺失率为15.15S。结论染色体异常和AZF微缺失是男性不育的重要原因,对男性不育诊断时有必要进行检查。Objective: To study the relationship between chromosome abnormalities and Y chromosome microdeletions of azoospermia factor (AZF) in males with infertility. Methods: A case-control study was conducted in 165 reproductive failure men with from our hospital of Chongqing. Chromosome Karyotype analysis was detected by Peripheral Blood chromosome G-band detection method. AZF microdeletions were further investigated in patients with Y chromosomal abnormalities by multiplex PCR amplification of 15 sequence-tagged sites. Results: Chromosomal abnormalities were found in 4 cases of 156 infertile patients. There were 1 case in 46XX female; 1 case in 47XXY; 1 case in 47XY+mar; 1 case in 46XY, y≥18 and 1case in 46XY, in (9) . The total rate of chromosomal abnormalities was 3.03%. Screening 165 blood samples STS marker of AZF regions showed AZF microdeletions in 25 (15.15%) patients. Conclusion: Chromosome abnormalities and AZF microdeletions are major factors for idiopathic infertile patients. Screening of Chromosome abnormalities and AZF gene microdeletion for idiopathic infertile patients is essential.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.222