不同凉粉草中天然苯甲酸的含量测定  

Determination of Natural Benzoic Acid in Different Mesona Chinensis Benth

作　　者：王海华 覃丽萍[2] 

机构地区：[1]南宁食品药品检验所,南宁530001 [2]广西右江民族医学院

出　　处：《中国药师》2014年第9期1493-1495,共3页China Pharmacist

基　　金：广西科学研究与技术开发计划项目(编号:桂科攻11107010-2-13);广西壮药质量标准(第二卷)标准研究项目(编号:MZY2010058)

摘　　要：目的：建立不同产地和存放时间的凉粉草中天然苯甲酸的含量测定方法。方法：采用高效液相色谱法,色谱柱为Agilent ZORBAX SB-C18（250 mm×4.6 mm,5μm）,以甲醇-0.02 mol·L-1的乙酸铵溶液为流动相（5∶95）;流速：1.0 ml·min-1;柱温：35℃;检测波长：230 nm;进样量：10μl。结果：苯甲酸在0.1~10.0μg·ml-1范围内线性关系良好,r=0.999 9;平均回收率在99.59%（RSD为1.57%,n=9）。数据表明不同产地凉粉草所含苯甲酸的含量不同;1号凉粉草的苯甲酸含量最多,为141.8 mg·kg-1,3号凉粉草的苯甲酸含量最少,为73.8 mg·kg-1,凉粉草打粉存放两年后苯甲酸的含量均明显下降。结论：该方法操作简单,准确性和重复性良好,为凉粉草资源的进一步深入研究提供依据,同时为其他植物中天然苯甲酸含量测定提供参考。Objective： To develop a method for the determination of natural benzoic acid in Mesona chinensis Benth. with different production places and storage time. Methods： HPLC was adopted,the column was an Agilent ZORBAX SB-C18（ 250 mm ×4.6 mm,5 μm） column,the mobile phase was methanol-0. 02 mol·L- 1ammonium acetate（ 5∶ 95） at the flow rate of 1. 0 ml·min- 1,the column temperature was 35 ℃,the detection wavelength was 230 nm,and the injection vocume was 10 μl. Results： Benzoic acid showed good linearity over the concentration range of 0. 1-10. 0 μg·ml- 1（ r =0.999 9）. The average recovery was 99.59%（ RSD =1.57%,n = 9）. Natural benzoic acid in powered Mesona chinensis Benth. was different because of various production places. The maximum content of natural benzoic acid in Mesona chinensis Benth. was 141. 8 mg·kg- 1,while the minimum content was 73.8 mg·kg- 1.The content of natural benzoic acid in Mesona chinensis Benth. stored for two years was declined obviously. Conclusion： The method with simple operation is accurate and reproducible. It can provide a basis for the further study of Mesona chinensis Benth. resources,and provide a reference for the content determination of natural benzoic acid in other plants.

关 键 词：凉粉草 苯甲酸 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]