口腔干预对慢性牙周炎大鼠颈动脉基质金属蛋白酶2,9和血清白细胞介素6的影响  

Effect of oral intervention on matrix metalloproteinase-2,9 expression in carotid arteries and serum interleukin-6 in rats with chronic periodontitis

作　　者：李洁婷[1] 刘玉娇[1] 任秀云[1] 常乐[1] 岳姿洁[1] 石学雪[1] 

机构地区：[1]山西医科大学口腔医学院牙周科,太原030001

出　　处：《中华口腔医学杂志》2014年第9期554-559,共6页Chinese Journal of Stomatology

基　　金：国家自然科学基金（81271144、31050002）;山西省自然科学基金（2010011050-1）

摘　　要：目的 模拟慢性牙周炎（chronic periodontitis,CP）患者临床治疗中的干预措施,研究不同口腔干预方式对CP大鼠动脉粥样硬化（atherosclerosis,As）发生及发展的影响,以期为牙周炎患者最佳治疗方案的制定提供参考.方法 用数字随机数字表法将50只SD大鼠随机分为A组（正常对照组,7只）、B组（As组,8只）、C组（CP组,35只）.建模成功后,再将C组分为C1组（自然进程）、C2组（牙周机械治疗）、C3组（药物治疗）、C4-1组（拔牙）和C4-2组（拔牙＋抗生素）,每组均为7只.按上述措施分别进行口腔干预,酶联免疫吸附法检测建模成功后1、3、5、7和9周血清白细胞介素6（interleukin-6,IL-6）的含量;免疫组化法检测颈动脉组织中基质金属蛋白酶2,9（matrixmetalloproteinase-2,9,MMP-2,9）的表达.结果 血清学结果显示：随着时间推移,B和C1组血清IL-6含量呈升高趋势,且显著高于A组（P＜0.01）;各干预组（C2、C3、C4-1、C4-2组）IL-6含量逐渐升高,并在建模成功后5周达峰值[C2、C3、C4-1、C4-2组分别为（62.3±14.3）、（58.2±8.7）、（127.0±29.9）及（120.6±23.1） ng/L],且均显著高于B组[（43.4±7.5） ng/L,P＜0.01],其中C4-1和C4-2组升高最明显,均接近B组的3倍,C2和C3组接近B组的1.5倍,之后逐渐下降.建模成功后9周,C2组[（28.6±8.1） ng/L、C3组[（40.8±15.1） ng/L]、C4-2组[（32.7± 11.1） ng/L]均显著低于B组[（66.3±7.8） ng/L]（P＜0.05）,且C2组最低;C4-1组（72.8±16.4）虽下降明显,但仍为最高.免疫组化结果显示：B、C1和C4-1组MMP-2,9大量阳性表达,且阳性染色强度均明显高于A组（MMP-2,9灰度值分别为：183.0±2.0、181.3±2.0）,灰度值差异有统计学意义（P＜0.01）,其中C4-1组阳性染色最强,B和C1组依次降低（MMP-2,9的灰度值分别为C4-1：123.1±2.9、121.0±3.2;B：126.4±2.0、124.8±2.8;C1：140.0±2.2、139.7±3.2）（P＜0.01）;C2、C3和C4-2组阳性染色强度均明�Objective To establish chronic periodontitis model in SD rats,and to investigate the effect of oral intervention on atherosclerosis.Methods Fifty male SD rats were randomly divided into three groups,group A（normal control）,group B（atherosclerosis,As） and group C（chronic periodontitis,CP）.Group C was further divided into group C1（natural process）,group C2（simple mechanical treatment）,group C3 （systemic antibiotics）,group C4-1（teeth extraction） and group C4-2（teeth extraction＋systemic antibiotics）,each group consisted of 7 rats.Every group received oral intervention.Serum interleukin（IL）-6 levels were detected in five different time points（1,3,5,7,9 weeks after a successful modeling） by enzyme linked immunosorbent assay.All animals were killed after 24 weeks.Matrix metalloproteinase（MMP）-2,9 in the proximal aorta was detected by immuno histochemistry.Results The levels of serum IL-6 in groups B and C1 increased gradually with time and became significantly higher than that in group A（P〈0.01）.Levels of serum IL-6 were increased gradually in each intervention group（C2,C3,C4-1,C4-2） and reached its peak at 5 weeks after modeling[C2：（62.3 ± 14.3） ng/L,C3：（58.2±8.7） ng/L,C4-1：（127.0±29.9） ng/L,C4-2：（120.6± 23.1） ng/L].Compared with group B,group C4-1 and C4-2 increased most significantly（P〈0.01）.Levels of serum IL-6 decreased gradually.Eventually,group C2[（28.6± 8.1） ng/L],C3[（40.8 ± 15.1） ng/L] and C4-2 [（32.7± 11.1） ng/L] were significantly lower than group B（P〈0.05）,and in group C2 IL-6 was the lowest.Although levels of serum of IL-6 significantly decreased in group C4-1[（72.8± 16.4） ng/L],but remained the highest.Immunohistochemistry showed that MMP-2,9 were expressed in group B,C 1 and C4-1,and significantly higher than in group A（183.0±2.0,181.3 ± 2.0）,the gray value differences were statistically significant（P〈0.01）.Group C4-1 （123.1 ±2.9,121.0± 3.2） was the strongest,group B （126

关 键 词：牙周炎 动脉粥样硬化 干预性研究 基质金属蛋白酶类 白细胞介素6 

分 类 号：R781.42[医药卫生—口腔医学]