LP17多肽对小鼠肠缺血再灌注损伤影响  

Effect of LP17 on intestinal ischemia-reperfusion injury of mice

作　　者：孙学童[1] 季涛[1] 张宗兵[2] 孔令尚[1] 刘牧林[2] 何先弟[3] 

机构地区：[1]蚌埠医学院胃肠外科,安徽省233030 [2]蚌埠医学院第一附属医院胃肠外科 [3]蚌埠医学院第一附属医院重症医学科

出　　处：《中华解剖与临床杂志》2014年第4期320-323,共4页Chinese Journal of Anatomy and Clinics

基　　金：基金项目：安徽省教育厅科研资助项目（2006kj103c）

摘　　要：目的：探讨LP17多肽对小鼠肠缺血再灌注（ IIR）损伤影响。方法将36只小鼠按数字表法随机分为假手术组（ S组）、IIR组（ I/R组）、IIR＋LP17对照肽治疗组（ C1组）、IIR＋LP17治疗组（ C2组）,每组9只。肠系膜上动脉夹闭20 min制作小鼠IIR模型,于再灌注前5 min将药物注入小鼠腹腔,24 h后处死小鼠,检测血清中可溶性髓样细胞触发受体-1（ sTREM-1）和肿瘤坏死因子-α（ TNF-α）的浓度,观察肠黏膜损伤程度,免疫组化法检测肝脏细胞的核因子-κB（ NF-κB）的表达情况。结果血清sTREM-1、血清TNF-α和肝脏细胞NF-κB在I/R组和C1组升高,分别为（1686.34±55.98）pg/ml、（121.81±8.01） pg/ml、（3.36±0.62）表达和（1643.73±65.45） pg/ml、（119.88±8.05）pg/ml、（3.21±0.94）分；在C2组降低,分别为（944.58±39.75）pg/ml、（65.92±4.91）pg/ml和（0.92±0.55）分,与I/R组和C1组比较,差异均有统计学意义（P值均〈0.01）。光镜下S组小鼠小肠黏膜基本正常；I/R组和C1组绒毛破坏较重,绒毛坏死明显,黏膜下水肿、炎性细胞浸润更加明显,肠壁各层均变薄；C2组肠绒毛损伤明显减轻,其肠黏膜损伤评分与其他各组比较,差异均有统计学意义（P值均〈0.01）。结论 LP17多肽通过调节TREM-1的信号转导通路,减轻NF-κB和TNF-α过度激活所致炎症反应,减轻IIR引起的肠黏膜及远隔脏器的损伤。Objective To investigate effect on intestinal ischemia-reperfusion injury of mice injected with LP17. Methods 36 mice were divided into three groups randomly（n =9 in each group）：Sham operation group（S group）, intestinal ischemia-reperfusion group （I/R group）, intestinal ischemia-reperfusion＋ LP17 control peptide group （ C1 group ） , intestinal ischemia-reperfusion ＋ LP17 treatment group （ C2 group） . Twenty min superior mesenteric artery occlusion in mice produced intestinal ischemia-reperfusion model, 5 min before reperfusion mice by intraperitoneal injection of the drug, 24 h after the mice were sacrificed and serum concentrations of triggering receptor expressed on myeloid cells （ sTREM-1 ） and tumor necrosis factor-α（ TNF-α） were observed mucosal the extent of damage, immune staining of liver cell expression of nuclear factor-κB（NF-κB）. Results The concentration of serum sTREM-1,the concentration of serum TNF-αand staining integral of the NF-κB shown on liver cell in I/R group（1 686. 34 ± 55. 98）pg/ml,（121. 81 ± 8. 01）pg/ml and （3. 36 ± 0. 62）and C1 group （1 643. 73 ± 65. 45）pg/m, （119. 88 ± 8. 05） pg/m, and 3. 21 ± 0. 94 were the highest and significantly reduced in the C2 group （944. 58 ± 39. 75） pg/ml,（65. 92 ± 4. 91） pg/ml,and 0. 92 ± 0. 55,compared with I/R group and C1 group, it was statistically significant（all P values〈0. 01）. The small intestine mucosa of the mice in S group was normal under light microscope. The villi in I/R group and C1 group were damaged seriously, villus necrosis significantly;those in C2 group received less damage and the difference had statically significance（all P values 〈0. 01）. Conclusions LP17 can reduce inflammatory response caused by over activation of TNF-αand NF-κB, and reduce the damage of the intestinal mucosa and the distant organ caused by intestinal ischemia-reperfusion injury through regulating the signaling pathway of TREM-1.

关 键 词：髓样细胞触发受体 LP17 肠缺血再灌注损伤 肠黏膜屏障 

分 类 号：R574[医药卫生—消化系统]