DNA损伤修复基因XRCC1启动子甲基化对肝癌易感性及其预后的影响  

Effect of DNA repair gene XRCC1 promoter methylation on the susceptibility and prognosis of hepatocelluar carcinoma

作　　者：方壮伟[1] 梁茱[1] 吴宁[1] 邱纯[1] 袁波[1] 彭永红[1] 周卫平[1] 谢贤和[1] 

机构地区：[1]海南省人民医院普外二科,海南海口570311

出　　处：《肝胆胰外科杂志》2014年第5期393-397,共5页Journal of Hepatopancreatobiliary Surgery

基　　金：海南省自然基金项目资助(808287)

摘　　要：目的探讨原发性肝癌组织和正常肝脏组织的DNA损伤修复基因XRCC1启动子甲基化状态,分析其与肝癌易感性的关系及对患者预后的影响。方法甲基化特异性PCR检测手术切除的肝癌组织78例及正常肝组织78例的XRCC1基因甲基化情况,并随访3年以上。结果肝癌组的XRCC1启动子甲基化率远高于对照组(P<0.05),肝癌组发生XRCC1启动子甲基化的危险是对照组的13倍(4.089 vs 41.332);XRCC1启动子甲基化的个体发生肝癌的危险是非甲基化的10.36倍(3.423 vs 31.354)(P<0.05);XRCC1启动子甲基化可致肝癌患者无进展期生存率和总体生存率降低(P<0.05)。结论 DNA损伤修复基因XRCC1启动子甲基化在肝癌发生和发展的过程中起着重要作用,并对肝癌患者较差的预后具有提示作用。Objective To explere DNA repair gene XRCC1 promoter methylation status of the hepatocellular carcinoma tissue and normal liver tissue,and to analyze its relationship with liver cancer susceptibility and the impact on its prognosis.Methods Methylation-specific PCR method was used for the detection of XRCC1 gene methylation status of 78 hepatocellular carcinoma tissues and 78 normal liver tissues,and ≥ 3 years of follow-up was conducted.Results XRCC 1 promoter methylation rates of HCC group was far higher than that of the normal tissues （P＜0.05）.XRCC1 promoter methylation could cause liver cancer progression-free survival and overall survival reduced （P＜0.05）.Conclusion DNA repair gene XRCC 1 promoter methylation plays an important role in the occurrence and development of liver cancer,and liver cancer patients with XRCC 1 promoter methylation can have poor prognosis.

关 键 词：肝癌 DNA损伤修复基因XRCC1 甲基化 易感性 预后 

分 类 号：R735.7[医药卫生—肿瘤]