Oct4在直肠癌组织中的表达及其临床意义  

Expression of Oct4 and its clinical significance in rectal cancer

作　　者：李兆顺[1] 尚培中[2] 谷华平[3] 苏红[3] 李伟[3] 

机构地区：[1]解放军251医院烧伤科,河北张家口075000 [2]解放军251医院普外科,河北张家口075000 [3]解放军251医院病理科,河北张家口075000

出　　处：《解放军医学杂志》2014年第9期699-702,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨Oct4在直肠癌组织和癌旁组织中的表达情况及其临床意义。方法采用免疫组化方法检测80例直肠癌组织、相应癌旁组织以及轻微慢性炎症患者黏膜组织中Oct4的表达情况,分析Oct4表达与临床病理参数之间的关系。同时在距肿物1、2、3cm处取黏膜组织作为手术切缘,分别标记为A、B、C组,以轻微慢性炎症患者黏膜组织为对照组(D组),分析与肿物不同距离处Oct4表达情况。结果 Oct4在直肠癌组织中的阳性表达率(52.50%)明显高于癌旁组织(30.00%,P<0.01)。Oct4的表达与组织学分化、TNM分期、肠周淋巴结转移有关(P<0.05),与患者性别、年龄、肿瘤直径、癌组织浸润深度及脉管癌栓无关(P>0.05)。手术切缘A组Oct4阳性表达22例(27.50%),B组18例(22.50%),C组7例(8.75%),D组2例(2.50%)。与C组和D组比较,A、B组Oct4表达均明显增加(P<0.05)。结论直肠癌组织中存在肿瘤干细胞的异常分化,Oct4过表达可为判断手术切缘状态提供一定参考。Objective To investigate the expression and the clinical significance of Oct4 in rectal carcinoma and cancer- adjacent tissues. Methods The expression of Oct4 in rectal carcinoma tissue and cancer-adjacent tissue obtained from 80 patients with rectal cancer, and the mucosa tissue from patients with mild chronic inflammation was detected by immunohistochemistry. The relationship between Oct4 expression and the pathological parameters was also analyzed. The cancer-adjacent mucosal tissue of Icm~ 2cm and 3cm away from the rectal cancer were selected and marked as groups A, B and C. The mucosal tissue from patients with mild chronic inflammation was selected as control （group D）. The expression of Oct4 of mucosa at different distance away from carcinoma was respectively analyzed. Results Oct4 was expressed in 52.50% rectal carcinoma, and the expression was higher than that in cancer-adjacent tissue （30.00%, P〈0.01）. The expression of Oct4 was related to histological malignant differentiation, TNM staging and lymph node metastasis （P〈0.05）, but it was not related to gender, age, tumor size, infiltration depth and vascular cancer embolus （P〉0.05）. Oct4 was expressed in 22 patients in group A （27.50%）, 18 in group B （22.50%）, 7 in group C （8.75%） and 2 in group D （2.50%）. Compared with groups C and D, the expression of Oct4 was significantly upregulated in groups A and B （P〈0.05）. Conclusion There is an abnormal differentiation of tumor stem cells in rectal cancer tissue. The existence of an over-expression of Oct4 may provide a reference for judging the margin of resection to certain extent.

关 键 词：直肠肿瘤 八聚体转录因子 病理学 外科 

分 类 号：R735.37[医药卫生—肿瘤]