成纤维细胞对肾癌细胞增殖迁移能力及依维莫司敏感性的影响  

Effects of fibroblasts on the proliferation and migratory capacity of renal cell carcinoma cell lines and sensitivity to everolimus

作　　者：宋佳佳[1] 卢永宁[1] 张进[1] 黄翼然[1] 

机构地区：[1]上海交通大学医学院附属仁济医院泌尿外科,200127

出　　处：《中华医学杂志》2014年第34期2704-2707,共4页National Medical Journal of China

基　　金：国家自然科学基金（81272841）;上海市自然科学基金（13ZR1425100）;上海市卫生计生委课题（2013SY027）志谢感谢中国科学院上海药物所提供技术支持

摘　　要：目的 观察肾癌细胞株786-O、Caki-1与正常人肾成纤维细胞（NHRF）相互作用后,肾癌细胞对依维莫司敏感性的变化.方法 建立肾癌细胞与成纤维细胞共培养体系,观察共培养后786-O、Caki-1的增殖及迁移能力[用吸光度（A）值表示]变化以及肾癌细胞对依维莫司敏感性的改变.结果 共培养后,786-O组肾癌细胞细胞增殖能力较对照组A值显著增加22％（2.35±0.05与1.93±0.15,P=0.01）,Caki-1组A值较对照组显著增加约80％（2.35±0.21与1.24±0.11,P=0.001）;迁移能力有所增强：786-O A值较对照组显著增加（1.34±0.12与0.86±0.04,P=0.03）,Caki-1 A值较对照组显著增加（1.53 ±0.11与0.98 ±0.11,P=0.04）.此外共培养组经依维莫司（1、5、10 μmol/L）处理后,在1μmol/L时,786-O组和对照组相比抑制率降低（24.05±5.05）％与（12.45±2.44）％,P=0.04,在5、10 μmol/L时抑制率无明显变化（P值分别为0.35、0.18）;Caki-1共培养组和对照组相比,抑制率均明显降低（49.02±2.17）％与（4.96±0.50）％,P=0.02;（52.67±2.57）％与（12.36±0.29）％,P=0.03;（60.87±3.87）％与（35.10±2.45）％,P=0.024.结论 NHRF促进肾癌细胞株786-O、Caki-1的增殖及迁移;NHRF参与786-O、Caki-1对依维莫司耐药现象的发生.Objective To observe the effects of normal human renal fibroblast （NHRF） on renal cell carcinoma cell lines.Methods Renal cell carcinoma （RCC） cell lines 786-O and Caki-1 were cocultured with NHRF for assessing the proliferation and migratory capacities of renal cell carcinoma cell lines and the sensitivity to everolimus.Results Co-culturing with NHRF substantially increased the proliferation capacity of both RCC cell lines （786-O：2.35 ± 0.05 vs1.93 ± 0.15,P =0.01 ; Caki-1：2.35 ± 0.21 vs 1.24 ± 0.11,P =0.001）.Similarly the migratory capacities of both cell lines became significantly enhanced after co-culturing （786-O：1.53 ± 0.11 vs 0.98 ± 0.11,P =0.04 ; Caki-1：1.53 ± 0.11 vs 0.98 ± 0.11,P =0.04） compared with untreated control.Furthermore,the sensitivities of both cell lines to everolimus （1,5,10 μmol/L） dramatically decreased in those pre-co-cultured with NHRF （786-O：P value 0.04,0.35,0.18） ; Caki-1：P value 0.02,0.03,0.024）.Conclusion NHRF promotes the proliferation and migration capacities of 786-O and Caki-1.And it may be involved in the resistance of RCC to everolimus.

关 键 词：癌 肾细胞 成纤维细胞 肿瘤微环境 耐药 

分 类 号：R737.11[医药卫生—肿瘤]