Wnt3a在毛囊及黑素细胞谱系中的表达  

Expression of Wnt3a in Hair Follicle and Melanocyte Lineage

作　　者：赵佳瑞[1] 白秀峰[2] 杨珂[3] 罗燕[2] 郭海英[2] 

机构地区：[1]第三军医大学学员旅4营,重庆400038 [2]第三军医大学基础部细胞生物学教研室,重庆400038 [3]重庆市干细胞治疗工程技术研究中心重庆医科大学附属儿童医院,重庆400014

出　　处：《现代生物医学进展》2014年第30期5814-5817,5832,共5页Progress in Modern Biomedicine

基　　金：国家自然科学青年基金项目(81301350);重庆市面上项目(cstc2013jcyjA10021)

摘　　要：目的:探讨毛囊周期中,Wnt3a在毛囊及黑素细胞中的表达变化。方法:以DCT-LacZ转基因小鼠为动物模型,通过X-gal染色技术观察黑素细胞谱系在小鼠皮肤中的分布情况;采用X-gal染色结合免疫组化方法检测Wnt3a在毛囊及黑素细胞谱系中的表达情况;采用RT-PCR方法对小鼠皮肤全层Wnt3a和TYR的mRNA表达进行半定量分析。结果:在生长期毛囊中,Wnt3a蛋白在表皮、毛囊外根鞘Bulge区、内根鞘以及毛球部均有表达,在黑素干细胞与黑素细胞也观察到Wnt3a;在退化期,Wnt3a的表达逐渐减弱,仅在外根鞘有较弱的表达,但黑素干细胞中没有观察到Wnt3a;在静止期,几乎检测不到Wnt3a的表达;TYR mRNA与Wnt3a mRNA在毛囊周期中的表达模式一致,在生长期最强,退化期减弱,静止期最弱。结论:Wnt3a可能对黑素细胞谱系分化起到促进作用。Objective： To examine the presence and location of Wnt3a in hair follicle and melanocyte lineage during hair cycle. Methods： By using Dct-LacZ transgenic mice, the protein expression pattern of Wnt3a was observed by X-gal staining and immunohistochemistry in the slides of skin in hair cycle. The mRNA expression quantity of Wnt3a and tyrosinase were observed by RT-PCR in the whole skin in hair cycle. Results： Wnt3a was expressed in the epidermis, bulge, IRS precursors and hair bulb in anagen hair follicle, decreased in catagen, and was basically not expressed in telogen. Melanocyte stem cells and melanocytes also expressed Wnt3a in anagen hair. The mRNA expression pattern of Wnt3a and tyrosinase were consistent, which were most strongly expressed in anagen, decreased in catagen, and lowest in telogen. Conclusion： Wnt3a may promote melanocyte lineage differentiation.

关 键 词：WNT3A 毛囊 黑素干细胞 黑素细胞 分化 

分 类 号：R-332[医药卫生] R322.99