牵张应力对成骨肉瘤细胞MG-63中RhoA和ROCK表达的影响  

Effects of Stretch Stress on RhoA and ROCK Expression in MG-63 Cells

作　　者：韩雨晨[1] 李菲菲[1] 杨磊[1] 李明伟[2] 刘余聪[1] 杨雪静[1] 冯雪[1] 

机构地区：[1]第四军医大学口腔医学院正畸科,陕西西安710032 [2]第四军医大学口腔医学院正牙体牙髓病科,陕西西安710032

出　　处：《现代生物医学进展》2014年第31期6011-6013,6018,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年科学基金项目(31200706)

摘　　要：目的:探索机械牵张应力对人成骨肉瘤细胞MG-63中RhoA/ROCK信号通路的影响。方法:将同一条件培养的细胞MG-63分为实验组(加力)与对照组(不加力),实验组采用Flexcell牵张应力加载系统,选择12%形变率作为加载应力值,分为五个时间组,分别加载1 h,4 h,8 h,12 h,24 h,RT-PCR检测RhoA、ROCK mRNA水平表达的变化,Western-Blot检测RhoA与Rock蛋白含量变化。结果:RT-PCR显示MG-63细胞受应力刺激后1 h RhoA、ROCK均未见明显变化(P>0.05),4小时后略有升高(P<0.05),在8 h达到最大值(P<0.05),12、24 h降低,但仍高于对照组(P<0.05);Western-Blot显示MG-63细胞受应力刺激后1 h RhoA、ROCK均未见明显变化,4 h仍未见明显变化,在8 h达到最大值,12、24 h降低,高于对照组。结论:机械牵张力加载下MG-63细胞内RhoA、ROCK表达升高并随时间增加出现峰值,提示其可能在成骨细胞力学信号转导过程中发挥重要作用。Objective： To investigate the effects of mechanical strain on RhoA and ROCK expression in osteoblasts MG-63. Methods： Mechanical strain at 12 % was applied to MG-63 cells for 0（control group）, 1, 4, 8, 12 and 24 hours respectively in vitro. RhoA and ROCK mRNA expression in the cells was examined by RT-PCR, RhoA and ROCK expressions were examined by Western Blot. Results： RT-PCR showed that mRNA expressions Of RhoA and ROCK were not significantly changed after 1 h strain treatment（P〉0.05） and slightly increased within 4 hours of mechanical strain application. Then the expression rose to the highest value after 8 hours and decreased after 12 and 24 hours （P〈0.05）. Western Blot represented a similar result. The protein expression was changed and up to the top after 8 hours then decreased gradually after 12 and 24 hours （P〈0.05）. Conclusion： Mechanical strain elongation regulates RhoA and ROCK expression of MG-63 cells and the expression was up to a maximum value with the increase of time. It was speculated that RhoA and ROCK may play an important role in cell signal transduction of mechanical strain.

关 键 词：MG-63 RHOA ROCK 机械牵张应力 表达 

分 类 号：R-33[医药卫生] R336