β-榄香烯对RAW264.7巨噬细胞源性泡沫细胞作用的研究  

Study on the Effects of β-elemene on RAW264.7 Macrophage-derived Foam Cells

作　　者：段文丽[1] 白仁仁[2] 钟颖[1] 徐进宜[2] 尚靖[1] 

机构地区：[1]中国药科大学新药筛选中心,江苏南京210009 [2]中国药科大学药物化学教研室,江苏南京210009

出　　处：《现代生物医学进展》2014年第31期6014-6018,共5页Progress in Modern Biomedicine

基　　金：国家科技重大专项"十二五重大新药创制"(2011ZX09401-007);"十二五"国家支撑项目(2012BAI30B01)

摘　　要：目的:研究β-榄香烯抗巨噬细胞源性泡沫细胞的形成及抑制巨噬细胞炎症因子分泌的作用。为探讨β-榄香烯抗动脉粥样硬化(AS)的作用提供依据。方法:采用氧化低密度脂蛋白(ox-LDL)诱导小鼠单核/巨噬细胞(RAW264.7)建立巨噬细胞源性泡沫细胞模型,采用油红O染色鉴定泡沫细胞形成。给予不同浓度(0.5,5,50μM)β-榄香烯干预后,ELISA方法检测巨噬细胞源性泡沫细胞内胆固醇含量和肿瘤坏死因子-α(TNF-α),白介素-6(IL-6)分泌量的变化。结果:β-榄香烯可降低巨噬细胞源性泡沫细胞内总胆固醇(P<0.05或P<0.01),胆固醇酯含量(P<0.01),减少炎症因子TNF-α,IL-6的分泌(P<0.05或P<0.01),并且呈现出一定的浓度依赖性。结论:β-榄香烯抑制巨噬细胞对ox-LDL的摄取,降低细胞内胆固醇的含量,抑制泡沫细胞的形成,同时改善巨噬细胞的炎症状态从而发挥抗动脉粥样硬化的作用。Objective： The effects of β-elemene on the generation of and inflammatory cytokine production from RAW264.7 macrophage-derived foam cells were observed. Methods： RAW264.7 macrophages were incubated with oxidized low-density lipoproteins （ox-LDL） or with both ox-LDL and β-elemene （0.5, 5, or 50 μM）. Oil red O staining was used to observe the formation of foam cells and the contents of cellular cholesterol, TNF-α and IL-6 were determined by commercially available kits. Results： Compared with the control cells, RAW264.7 macrophage-derived foam cells showed significantly increased contents of total cholesterol,cholesterol ester, TNF-α and IL-6 （P〈0.01 or P〈0.001）. However, β-elemene treatments significantly lowered the contents of total cholesterol, cholesterol ester and decreased secretion of TNF-α and IL-6 in a dose-dependent manner （P〈0.05 or P〈0.01）. Conclusion： β-elemene can be involved in the intake of ox-LDL, decrease the contents of total cholesterol and cholesterol esters,reduce the secretion of inflammatory cytokines in the macrophages, inhibit the formation of foam cell and produce the anti-atherosclerotic effect.

关 键 词：动脉粥样硬化 巨噬细胞 胆固醇 胆固醇酯 IL-6 TNF-旺 

分 类 号：R-33[医药卫生] R543.5