p38-2G4蛋白高表达对小鼠红白血病细胞增殖和分化的影响  

Effects of p38-2G4 high-expression on the proliferation and erythriod differentiateon of murine erythroleukemia cells

作　　者：朱晓芳[1] 施鸣铭[1] 杨祖立[1] 赵辅昆[1] 张世馥[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《解剖学报》2014年第5期670-674,共5页Acta Anatomica Sinica

摘　　要：目的探讨小鼠增殖相关蛋白p38-2G4基因表达上调对小鼠红白血病(MEL)细胞增殖和诱导分化能力的影响。方法构建p38-2G4-pLJM1高表达重组载体与pCMV-VSV-G和pCMV-dR8.2包装质粒共转染HEK293T细胞慢病毒,感染MEL细胞,建立稳定高表达p38-2G4蛋白的MEL细胞系,Western blotting检测稳定株p38-2G4蛋白的表达。MTT实验和联苯胺染色法检测p38-2G4蛋白高表达对MEL细胞增殖和诱导分化能力的影响。结果高表达稳定株中p38-2G4蛋白表达量明显高于对照组细胞(P<0.05)。p38-2G4蛋白的高表达能显著影响MEL诱导分化过程,导致血红蛋白合成减少(P<0.05),但不能明显改变细胞活力(P>0.05)。结论 p38-2G4蛋白的高表达对MEL细胞增殖无明显影响,但能显著影响其被丁酸钠诱导分化的过程。Objective To explore the effect of mouse proliferation-associated protein 2G4 （p38-2G4） high-expression on the proliferation and erythriod differentiation of murine erythroleukemia （ MEL ） cells.Methods To establish the recombinant lentivirus vector p 38-2G4-pLJM1, the p38-2G4-pLJM1 was cotransfected into HEK293T cells to obtain lentivirus with pCMV-VSV-G and pCMV-dR8.2.Lentivirus were infected into MEL cells to establish the stably p 38-2G4 high-expressed MEL cells.Western blotting was used to analyse the high-expression efficiency.MTT assay and benzidine staining were applied to detect the cell viability and hemoglobin synthesis of the stable cell line in presence /absence of inducers.Results Western blotting showed that the p38-2G4 high-expression stable cell strain had a higher expression of p38-2G4 as compared to the control group （ MEL） （ P 〈0.05）.MTT result showed that there was no difference between the p38-2G4 high-expression cell strain and the control group （P〉0.05）, but the hemoglobin synthesis had been reduced as compared to the control group （P〈0.05）.Conclusion p38-2G4 high-expression does not affect the cell viability of MEL cells , but inhibits the erythriod differentiation of MEL cells in three independent experiments .

关 键 词：p38-2G4 小鼠红白血病 慢病毒转染 高表达 免疫印迹法 小鼠 

分 类 号：R733.7[医药卫生—肿瘤]