PPAR-γ配体对绒癌细胞系JEG-3体外增殖、分泌和侵袭能力的影响  

The effect of PPAR-γ ligand on proliferation and secretion and invasion of JEG-3 choriocarnoma cells in vitro

作　　者：丁峰[1] 崔新红[1] 钮彬[1] 邢福祺[2] 

机构地区：[1]解放军第456医院妇产科,山东济南250031 [2]南方医科大学附属南方医院生殖中心,广东广州510515

出　　处：《基础医学与临床》2014年第10期1344-1347,共4页Basic and Clinical Medicine

基　　金：国家科技部"973"课题(G1999055903)

摘　　要：目的观察PPAR-γ配体吡格列酮(PGZ)调节绒癌细胞系JEG-3的增殖、分泌h CG和体外侵袭、转移能力。方法用MTT法和IEMA测定细胞的增殖和分泌h CG。Matrigel侵袭模型分析细胞的迁徙和侵袭能力。结果小剂量PGZ对JEG-3有增殖抑制作用,随PGZ浓度的增加,JEG-3透过Matrigel膜的细胞数明显减少(P<0.01)。结论PGZ明显抑制JEG-3肿瘤细胞的生长和侵袭能力,提示PPAR-γ途径可能是妊娠滋养细胞肿瘤治疗的新方向。Objective To observe the effect of ligand of peroxisome proliferators-activated receptor gamma （PPAR gamma） on the proliferation and secretion of trophoblast and choriocarnoma cells （JEG-3） and to study the role played by PPAR gamma during the process of JEG-3 activation.Methods We employed MTT assay to observ the growth of the cells and IEMA the secretion of hCG in the cells.Matrigel invision chambers were performed to analyse the cell migration and invasion.Results The proliferation of JEG-3 was suppressed by PGZ with a low dosedependent manner.The numbers of JEG-3 was obviously inhibited by PGZ（P ＜ 0.01）with increasing concentration.Conclusions The results obtained with this in vitro system demonstrated that PPAR gamma activation may inhibit JEG-3 proliferation and invasiveness.PPAR gamma may be a new therapeutic target for gestational trophoblastic tumor in humans.

关 键 词：妊娠滋养细胞疾病 过氧化物酶增殖体激活受体 增殖 绒毛膜促性腺激素 肿瘤侵袭力 

分 类 号：R737.33[医药卫生—肿瘤]