PTTG1通过上皮-间质转化促进非小细胞肺癌的迁移和侵袭  

PTTG1 induces migration and invasion of non-small cell lung cancer through epithelial-mesenchymal transition

作　　者：李洪利[1] 尹崇高[2] 

机构地区：[1]潍坊医学院医学研究实验中心,山东潍坊261053 [2]潍坊医学院护理学院,山东潍坊261053

出　　处：《基础医学与临床》2014年第10期1376-1380,共5页Basic and Clinical Medicine

基　　金：山东省中青年科学家科研基金(BS2011YY060);山东省高等学校科技计划(J10LF64;J12LK03;J13LK03)

摘　　要：目的研究PTTG1在非小细胞肺癌侵袭转移中的作用。方法应用免疫组织化学染色法检测80例NSCLC组织中PTTG1、E-cadherin和Vimentin的表达,并分析其相关性。细胞分组:1)A549/con,转染空载的A549,2)A549/PTTG1,转染目的基因的A549,3)Scr/H1299,转染空载的H1299,4)Si PTTG1/H1299,敲除PTTG1的H1299;用Western blot检测细胞中PTTG1、E-cadherin、Vimentin蛋白的表达情况。结果 NSCLC组织中PTTG1蛋白的阳性表达率为85%与E-cadherin的表达呈负相关(P<0.05)而与Vimentin的表达呈正相关(P<0.05)。Western blot结果显示在Si PTTG1/H1299细胞中PTTG1蛋白表达水平明显降低,Vimentin蛋白表达水平降低而E-cadherin蛋白表达水平升高。同时在A549/PTTG1细胞中Vimentin蛋白表达水平升高而E-cadherin蛋白表达水平降低。结论PTTG1能促进NSCLC EMT的发生,从而促进NSCLC的侵袭和转移。Objective To investigate the role of PTTG1 in the migration and invasion of Non-small cell lung cancer.Methods To detect the expression of PTTG1,E-cadherin and Vimentin in 80 cases of NSCLC using immunohistochemistry.Cells were divided into four groups：1）A549/con,cells were transfected by empty vector; 2） A549/PTTG1,cells were transfected by purpose gene; 3）Scr/H1299,cells were transfected by empty vector; 4）SiPTTG1/H1299,cells were knocked down purpose gene.Western blot was used to analyze the expression of PTTG1,E-cadherin and Vimentin.Results The protein expression of PTTG1 in NSCLC was negatively correlated with E-cadherin and positively correlated with Vimentin （P ＜ 0.05）.H1299 cells expressed higher PTTG1,and A549 cells expressed lower PTTG1.The expressions of PTTG1 and Vimentin were lower in SiPTTG1/H1299 cells than those in Scr/H1299 and H1299 cells after trancfected 24 hours,but the E-cadherin expression was higher.At the same time,the protein expression of Vimentin was up-regulated and the expression of E-cadherin was down-regulated in A549/PTTG1 cells.Conclusions PTTG1 promotes migration and invasion of NSCLC through EMT.

关 键 词：垂体肿瘤转化基因1 上皮-间质转化 肺肿瘤 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]