载脂蛋白E基因多态性对星形胶质细胞致伤后早期胞内p38MAPK表达的影响  

Effect of apolipoprotein E polymorphisms on expression of p38MAPK in the early period of astrocytes injury

作　　者：吴海涛[1] 周帅 夏海坚[3] 王玉玉[1] 王培[1] 孙晓川[3] 

机构地区：[1]遵义医学院附属医院神经外科,563000 [2]云南省人民医院神经外科 [3]重庆医科大学附属第一医院神经外科

出　　处：《中华创伤杂志》2014年第9期962-965,共4页Chinese Journal of Trauma

基　　金：贵州省科学技术基金资助项目（黔科合[2013] 2332号）

摘　　要：目的 探讨载脂蛋白E基因（apolipoprotein E,APOE）与星形胶质细胞致伤后早期胞内p38分裂原激活的蛋白激酶（p38 mitogen activated protein kinase,p38MAPK）表达的相关性.方法 （1）建立APOEε2、APOEε3及APOEε4三种基因型星形胶质细胞划痕损伤模型;（2）于致伤前、伤后12h、24h、48 h及72 h时相点,用RT-PCR检测各基因型细胞内p38MAPK mRNA的表达情况;（3）在相应时相点用Western-blot检测各组细胞内p38MAPK蛋白的表达情况. 结果 各型细胞相对于自身对照（致伤前）,p38MAPK的表达随时间呈逐渐升高趋势,但致伤前及伤后12 h,三型细胞两两间进行比较,其p38MAPK的表达差异不显著（P＞0.05）;伤后24,48及72 h,三型细胞p38 MAPK的表达进行性增加,且ε4组明显高于ε2和ε3组（P＜0.05）. 结论 损伤后早期,携带APOEε4等位基因的星形胶质细胞内p38MAPK的表达高于ε2和ε3型,提示ε4携带体伤后由p38MAPK信号通路介导的炎性损伤要重于其他两型,由此导致急性期伤情加重及不良预后.Objective To investigate the correlation between apolipoprotein E （APOE） polymorphisms and expression of p38MAPK of injured astrocytes in the early stage.Methods Scratch injury to astrocytes of three different alleles of APOE （ε2,ε3,andε4） was induced.RT-PCR and Western-blot were applied to detect dynamic changes of intracellular p38MAPK before injury and at 12,24,48,and 72 hours postinjury.Results Expression of p38MAPK in APOEε2,ε3,andε4 astrocytes increased gradually over time,whereas before and 12-hour after injury,the difference was insignificant in pair comparison （P ＞ 0.05）.p38MAPK in APOEε2,ε3,andε4 astrocytes revealed progressive up-regulation at 24,48,and 72 hours postinjury,but the expression in APOEε4 astrocytes was the highest （P ＜ 0.05）.Conclusion In the early period of injury,highly expressed p38MAPK in APOEε4 astrocytes indicates a more active p38MAPK-induced inflammatory response in APOEε4 carriers which may contribute to acute exacerbation and poor outcome.

关 键 词：星形细胞 载脂蛋白类 基因多态性 p38分裂激原激活的蛋白激酶 

分 类 号：R363[医药卫生—病理学]