IGSF4基因在非小细胞肺癌中甲基化状态及其意义研究  

Study on methylation status of gene IGSF4 in NSCLC and significance

作　　者：朱剑武[1] 李梦侠[1] 王东[1] 高天[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆400042 [2]重庆医科大学附属第一医院妇产科

出　　处：《西南国防医药》2014年第9期932-935,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：重庆市自然科学基金(CSTC2010BB5198)

摘　　要：目的通过MassARRAY质谱甲基化测序检测,分析IGSF4基因的启动子区在非小细胞肺癌(NSCLC)中的甲基化修饰规律和特点,进一步探讨NSCLC早期诊断新方法。方法首先利用MassARRAY质谱甲基化测序及甲基化特异PCR(MSP)观察该基因在NSCLC中的甲基化状态,再通过RT-PCR验证其在NSCLC中表达情况。结果 MassARRAY质谱甲基化测序方法对7例NSCLC和3例正常对照血样检测结果显示,IGSF4基因启动子区8个CpG位点在NSCLC中甲基化程度明显增高(P<0.01),呈高度甲基化状态;MSP结果亦显示,IGSF4基因在NSCLC中与正常外周血比较呈高甲基化;在细胞水平上进一步验证IGSF4基因表达情况,结果发现该基因在NSCLC中呈低表达。结论 IGSF4基因在NSCLC中呈高甲基化状态,在NSCLC中低表达,可作为无创性早期诊断NSCLC的新方法。Objective To analyze the regulation and characteristics of methylation of the promoter region of the gene IGSF4 in NSCLC with MassARRAY Epityper for a further study of new early diagnostic method of NSCLC.Methods MassARRAY Epityper and methylation-specific PCR（MSP） were applied to observe the methylation status of the gene in NSCLC and the expression of the gene in NSCLC were verified by real-time PCR.Results The detection results of blood samples from 7 cases with NSCLC and 3 normal controllers by MassARRAY Epityper revealed a hypermethylation of 8 CpG sites in the promoter region of IGSF4 （P ＜ 0.01） ; the results of MSP also showed a hypermethylation of IGSF4 in NSCLC;the result of real-time PCR showed a lower level of IGSF4 in NSCLC patients than the normal control in the cellular level.Conclusions IGSF4 is in hypermethylation status and low expression in cellular level in NSCLC,which may serve as a new method of noninvasive early diagnosis of NSCLC.

关 键 词：非小细胞肺癌 DNA甲基化 IGSF4 A549细胞株 MSP 

分 类 号：R734.2[医药卫生—肿瘤]