β-甘露聚糖酶对豆粕、棉粕和菜粕的体外酶解效果研究  

Effect of enzymatic hydrolysis in vitro for β-mannanase in soybean meal, cottonseed meal and rapeseed meal

作　　者：田军辉[1] 袁林[1] 乔家运[2] 王志祥[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]天津市畜牧兽医研究所,天津300381

出　　处：《饲料工业》2014年第18期20-23,共4页Feed Industry

基　　金：国家科技支撑计划项目"新型饲料用酶制剂创制与应用[2013BAD10B01]"

摘　　要：试验旨在通过体外消化试验来研究β-甘露聚糖酶对豆粕、棉粕及菜粕的酶解效果。采用单因子试验设计,设Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ5个组,每组设4个重复,其中Ⅰ组为对照组,Ⅱ、Ⅲ、Ⅳ和Ⅴ组为试验组,各组β-甘露聚糖酶添加水平分别为0、0.4%、0.8%、1.6%和2.4%。结果表明:1豆粕中Ⅲ、Ⅳ、Ⅴ组可溶性甘露糖含量比对照组分别增加了3.0%、3.9%、4.6%,差异显著(P<0.05),Ⅱ组与对照组相比较差异不显著(P>0.05);Ⅲ、Ⅳ、Ⅴ组可溶性葡萄糖含量较对照组分别增加了4.3%、4.9%、6.1%,差异显著(P<0.05),Ⅱ组与对照组相比较差异不显著(P>0.05)。2棉粕中Ⅲ、Ⅳ、Ⅴ组可溶性甘露糖含量比对照组分别增加了7.4%、8.6%、8.9%,差异显著(P<0.05),Ⅱ组与对照组相比差异不显著(P>0.05);Ⅳ、Ⅴ组可溶性葡萄糖含量比对照组分别增加了9.1%、10.5%,差异显著(P<0.05),Ⅴ组可溶性葡萄糖含量比Ⅱ组增加了6.3%,差异显著(P<0.05),Ⅱ、Ⅲ组与对照组相比差异均不显著(P>0.05)。3菜粕中Ⅳ、Ⅴ组可溶性甘露糖含量比对照组分别增加了6.6%、9.5%,差异显著(P<0.05),Ⅲ、Ⅱ组与对照组相比较差异均不显著(P>0.05);Ⅲ、Ⅳ、Ⅴ组可溶性葡萄糖含量比对照组分别增加了12.2%、14.6%、16.7%,差异显著(P<0.05),Ⅱ组与对照组差异不显著(P>0.05)。试验结果表明,豆粕、棉粕及菜粕中β-甘露聚糖酶的适宜添加水平分别为0.8%、0.8%和1.6%。The trial was conducted to study theeffect of enzymatic hydrolysis in vitro for β- mannanase in soybean meal, cottonseed meal and rapeseed meal. Using single factor design method the experiment was divided into 5 groups （Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ ） with 4 replicates that were supplemented with 0, 0.4%,0.8%, 1.6%, and 2.4% β-mannanase respectively, and the control group was defined to Ⅰ. The results showed that： ① Comparing with the control group the quantities of the soluble mannose of Ⅲ, Ⅳ and Ⅴ were increased significantly by 3.0%, 3.9%, and 4.6% in soybean meal respectively （P〈0.05）, but there was no significant effect between Ⅱ and the control group （P〉0.05）. The quantities of the sol- uble glucose of ⅢⅣ and Ⅴ were improved significantly by 4.3%, 4.9%, and 6.1% respectively compared to the control group in soybean meal （P〈0.05）, but there was no significant difference between Ⅱ and the control group （P〉0.05）. ② Comparing with the control group, the quantities of the soluble mannose of Ⅲ, Ⅳ and Ⅴ were increased significantly by 7.4%, 8.6%, and 8.9% in cottonseed meal respectively （P〈0.05）, but there was no significant effect between Ⅱ and the control group （P〉0.05）. The quantities of the soluble glucose of Ⅳ and Ⅴ were improved significantly by 9.1% and 10.5% compared to the control group in cottonseed meal respectively （P〈0.05）, but there was no significant difference among Ⅲ, Ⅱand the control group （P〉0.05）. ③ Comparing with the control group, the quantities of the soluble mannose of Ⅳ and Ⅴ were increased significantly by 6.6% and 9.5% in rapeseed meal respectively （P〈0.05）, but there was no significant effect among Ⅲ, Ⅱ and the control group （P〉0.05）. The quantities of the soluble glucose of Ⅲ, Ⅳ and V were improved significantly by 12.2%, 14.6% and 16.7% compared to the control group in rapeseed meal respectively （P〈0.05）, but there was no significant difference between Ⅱ and the c

关 键 词：Β-甘露聚糖酶 豆粕 棉粕 菜粕 体外酶解 

分 类 号：S816.32[农业科学—饲料科学]