微小RNA-487a调节乳腺癌耐药细胞药物敏感性的作用与机制研究  

The effects and mechanisms of miR-487a on the drug sensitivity of breast cancer resistance cells

作　　者：江茜[1] 马孟涛 何苗[1] 王岩[1] 焦旭阳[1] 赵琳[1] 于兆进[1] 孙明立[1] 魏敏杰[1] 

机构地区：[1]中国医科大学药学院,沈阳110001

出　　处：《山西医药杂志》2014年第16期1879-1882,共4页Shanxi Medical Journal

基　　金：国家自然科学基金(81102472;81173092);沈阳市科技计划项目(F12-148-9-00)

摘　　要：目的探讨miR-487a调节米托蒽醌(MX)耐药的乳腺癌耐药细胞MCF-7/MX对MX敏感性的作用与机制。方法通过在乳腺癌耐药细胞MCF-7/MX中转染miR-487amimic构建miR-487a高表达乳腺癌耐药细胞模型,应用(qRT)-PCR、蛋白印迹法、流式等方法检测miR-487a通过靶向作用乳腺癌耐药蛋白BCRP增加乳腺癌细胞对MX的敏感性。结果在MCF-7/MX细胞中miR-487a高表达抑制BCRP的表达,增加MX的细胞内蓄积,提高该细胞对MX的敏感性。结论 miR-487a通过靶向作用BCRP,增加乳腺癌细胞对MX的敏感性。Objective To explore the mechanisms of miR-487 aregulating the sensitivity of breast cancer resistant cells MCF-7/MX to MX.Methods miR-487 amimic was transfected in breast cancer resistant cells MCF-7/MX to build cell models that overexpress miR-487 a.The expression of miR-487 aand breast cancer resistance protein（BCRP）mRNA was measured by qRT-PCR,and the protein expression and transport function of BCRP for MX after the transfection of miR-487 amimic in MCF-7/MX cells were examined by Western blotting and flow cytometry analysis.Results The overexpression of miR-487 ainhibited the mRNA and protein expression of BCRP in MCF-7/MX cells,increased the accumulation of MX,and improved the sensitivity of MCF-7/MX cells to MX.Conclusion miR-487 aincreased the sensitivity of breast cancer cells to MX by targeting BCRP.

关 键 词：乳腺肿瘤 抗药性 肿瘤 miR-487a 药物敏感性 MCF-7/MX 

分 类 号：R737.9[医药卫生—肿瘤]