缺氧缺血性脑病新生儿血浆高迁移率族蛋白1和细胞间黏附分子-1表达水平及其与新生儿神经行为评分的相关性  

Relationship between high mobility group box 1 protein,intercellular adhesion molecule-1 and neonatal behavioral neurological assessment in neonates with hypoxic-ischemic encephalopathy

作　　者：黄群[1] 谭丹凤[1] 马敏[1] 黄广文[1] 龙小兰[1] 吴虹[1] 

机构地区：[1]湖南省妇幼保健院儿童保健科,410008

出　　处：《中国神经免疫学和神经病学杂志》2014年第5期368-371,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：湖南省妇幼保健院科研基金资助项目(No:SFY201309)

摘　　要：目的探讨高迁移率族蛋白1(high mobility group box 1protein,HMGB1)和细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)在缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)新生儿血浆中的表达水平及其与新生儿神经行为(neonatal behavioral neurological assessment,NBNA)评分的相关性。方法收集30例正常新生儿和30例HIE患儿血液标本,分离血浆,采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血浆HMGB1和ICAM-1浓度。并由受过专业培训的新生儿医师对其进行NBNA评分。结果与正常对照组相比,HIE患儿血浆ICAM-1和HMGB1水平显著升高(P<0.01);HIE患儿血浆HMGB1与ICAM-1呈正相关(r=0.854,P<0.01),而ICAM-1、HMGB1水平与NBNA评分呈负相关(分别为r=-0.851,P<0.01;r=-0.871,P<0.01)。结论 HIE患儿血浆HMGB1、ICAM-1浓度较非HIE新生儿明显升高且与HIE病情轻重密切相关,提示HMGB1、ICAM-1可能在新生儿HIE的发生发展中发挥重要作用。Objective To explore the expression and correlation of high mobility group box 1 protein （HMGB1）,intercellular adhesion molecule-1 （ICAM-1） and neonatal behavioral neurological assessment （NBNA） in hypoxic-ischemic encephalopathy （HIE） neonates.Methods Thirty controls and 30 HIE neonates were recruited in our study.Plasma was prepared for detecting the HMGB1,ICAM-1 concentrations using ELISA kit.The NBNA score was determined by a specially trained physician.Results Comparing with controls,ICAM-1,and HMGB1 concentrations in plasma were significantly increased （P＜0.01）.Furthermore,there was a positive correlation between HMGB1 and ICAM-1 in HIE group （r=0.854,P＜0.01）.ICAM-1,HMGB1 levels and NBNA score showed significant negative correlations respectively （r=-0.851,P＜0.01; r=-0.871,P＜0.01） Conclusions The serum level of HMGB1,ICAM-1 in HIE patients are obviously higher than non-HIE neonates and are closely related to the severity of HIE,which may play an important role in the pathophysiology process of HIE.

关 键 词：缺氧缺血 脑 婴儿 新生 高迁移率族蛋白类 胞间黏附分子1 神经行为学表现 

分 类 号：R742.12[医药卫生—神经病学与精神病学]