偶氮胂Ⅲ-β-环糊精包合物与DNA作用方式的光谱法研究  

Spectroscopic study on the mechanism of interaction between arsenazo Ⅲ-β-cyclodextrin and DNA

作　　者：罗春媚[1] 张丽[1] 王兴明[1] 杨欢[1] 

机构地区：[1]西南科技大学材料科学与工程学院化学系,绵阳621010

出　　处：《四川大学学报（自然科学版）》2014年第5期1005-1009,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30973634)

摘　　要：在pH=7.40的Tris-HCl缓冲溶液中,采用紫外光、荧光光谱法和粘度法研究了偶氮胂-β-环糊精包合物(β-CD-AS)与鲱鱼精DNA的作用方式.用摩尔比法确定了β-CD-AS与DNA的结合比为5:1.通过热力学研究得出结合常数为K291.15K=8.68×105 L·mol-1,K310.15K=8.43×105 L·mol-1.热力学函数为ΔrHmθ=27.04J·mol-1,ΔrGmθ291.15K=-2.78×104 J·mol-1,ΔrSmθ=95.47J·mol-1·K-1.结果显示该反应为熵驱动.实验进一步表明β-CD-AS与DNA之间的作用方式为混合模式,即部分嵌插和沟渠作用.The mechanism of interaction between arsenazo Ⅲ-β-cyclodextrin and DNA has been studied by UV-visible spectra, fluorescence spectra and viscosity measurements in solution of Tris-HCl （pH = 7. 4）. From mole ration method, the mole ration between arsenazo Ⅲ-β-cyclodextrin and DNA is found to be 5 ： 1. The thermodynamic studies show that the binding constants of arsenazo Ⅲ-β-cyclodextrin interaction with DNA are K291.15K=8. 68 × 105 L· mol-1 and K310.15K =8.43 × 105 L ·mol-1, and the corre sponding thermodynamic parameters are △rHθm=27.04J· mol-1,△rGθm291.15K=-2.78×104 J·mol-1,△rSθm=95.47J· mol-1·K-1. The results suggest that entropy is the driven force in this reaction. The interaction of arsenazo Ⅲ-β-cyclodextrin and DNA is through groove interaction and partial intercalation method.

关 键 词：鲱鱼精DNA 偶氮胂(Ⅲ) 分子探针 Β-环糊精 作用方式 

分 类 号：O629.74[理学—有机化学] O629.113[理学—化学]