不同方法下过敏性紫癜患者IgA型抗中性粒胞浆抗体的表达和意义  

The expression and clinic value on IgA and IgG antineutrophil cytoplasmic antibody of patients with anaphylactoid purpura

作　　者：邓茂[1] 黎昌强[1] 廖勇梅[1] 

机构地区：[1]泸州医学院附属医院,四川泸州646000

出　　处：《现代预防医学》2014年第17期3249-3250,3258,共3页Modern Preventive Medicine

基　　金：四川省卫生厅项目(100286)

摘　　要：目的探究不同方法检测Ig A型抗中性粒细胞胞浆抗体在过敏性紫癜患者中的表达及与临床的关系。方法对120例过敏性紫癜患者的血清分别采用酶免疫法(ELISA)和间接免疫荧光法(IIF)检测Ig A-ANCA,分析检测结果与敏性紫癜的关系。结果 ELISA法检出Ig A-p ANCA阳性38例(31.67%),其中肾性紫癜及有肾损害的混合型紫癜26例,1例有痛风。IIF法检出Ig A-p ANCA阳性7例,均为肾性紫癜。两组比较差异有统计学意义(P<0.05)。对Ig A-p ANCA阳性患者经治疗后1年再次检测,Ig A-p ANCA阳性的主要是肾性紫癜。结论采用2种不同方法检测的过敏性紫癜患者Ig A-p ANCA结果差异较大,可能与其他原因引起ELISA法检测结果呈假阳性有关。不过两种方法均证明Ig A-p ANCA与肾性紫癜的发病有一定关系。Objective To explore the expression and clinical relationship of Ig A antineutrophil cytoplasmic antibody of patients with anaphylactoid purpura by different methods. Methods Detected Ig A antineutrophil cytoplasmic antibody of 120 patients with anaphylactoid purpura by enzyme-linked immunosorbent assay（ELISA） and indirect immunofluorescence（IIF）. Results The Ig A-p ANCAs of 38 cases（31.67%） were positive tested by ELISA, including 26 cases had renal purpura and renal damage of mixed purpura and 1 case had gout. The Ig A-p ANCAs of 7 cases（5.83%） were all positive by IIF detection, and all patients had renal purpura. There was a statistical significance（P〈0.05）. One year after treatment, the test results showed the positive Ig A-p ANCA was still concentrated in the patients with renal purpura. Conclusion The results of Ig A-p ANCA in the patients with anaphylactoid purpura detected by the two different methods have significant differences, and it may be linked to the causes that induce the false positive result of ELISA method. But the two methods prove that Ig A-p ANCA has a relationship with the renal purpura.

关 键 词：IG A抗中性粒细胞胞浆抗体 酶免疫法 间接免疫荧光法 过敏性 紫癜 表达 

分 类 号：R113[医药卫生—公共卫生与预防医学]