固相萃取-加压毛细管电色谱法测定水产品接触材料中五种芳香胺  

Determination of the Five Aromatic Amines in Seafood Contact Materials by Solid-phase Extraction-pressurized Capillary Electrochromatography

作　　者：罗雨阳 吴伟建[1] 杨立业[1] 欧阳小琨[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316022

出　　处：《浙江海洋学院学报（自然科学版）》2014年第2期165-170,共6页Journal of Zhejiang Ocean University(Natural Science Edition)

基　　金：浙江省自然科学基金项目(Y2111223;Z3110487);浙江省科技厅公益项目(2012C24027)

摘　　要：建立了一种固相萃取-加压毛细管电色谱同时测定水产品接触材料中5种芳香胺含量的方法。采用EP-100-20/45-3-C18毛细管柱和紫外检测器进行分离检测，以甲醇：磷酸氢二钾水溶液（20mmol，pH=8）为流动相，采用梯度洗脱，在240nm波长下加压＋6kV，五种芳香胺得到良好的分离。五种芳香胺在2～20mg/L浓度范围内线性良好，相关系数均大于0.99；加标回收率在70.1%～84.5%之间，RSD〈5.7%，检出限介于1.0～4.0mg/L（S/N=3）之间。该方法灵敏度较好，前处理简便易行，适用于水产品接触材料中芳香胺的快速检测。In this paper, a validated pCEC method has been developed for the separation and qualification of five aromatic amines in seafood contact matenals. By combining solid-phase extraction, these amines were efficiently separated on an EP-100-20/45-3-C18 capillary column and UV detector, using a mobile phase of methanol/phosphate buffer solution （20 mmol/L, pH=8） in gradient elution. Flow-rate, detection wavelength, temperature, and impressed voltage were adjusted to 0.04 mL/min, 240 nm, room temperature, and ＋6 kV, re-spectively. The liner range was 2-20 mg/L, the correlation coefficient were＆gt;0.99, and the absolute recoveries ranged from 70.1% to 84.5% for the five compounds. RSDs of the five aromatic amines were less than 5.7%, and the limit of detection was between 1.0-4.0 mg/L （S/N=3）. The method is suitable for the rapid detection of the aromatic amines which is sensitive and convenient.

关 键 词：毛细管电色谱 芳香胺 水产品 接触材料 

分 类 号：O657.7[理学—分析化学]