Flt3和c-kit在脐血CD34^+干/祖细胞中功能性表达及意义  

Functional Expression of Flt3 and c-kit on Cord Blood CD34+ Stem/Progenitor Cells and Its Significance

作　　者：马艳萍[1] 邹萍[1] 肖娟[1] 黄世昂[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液科,武汉430022

出　　处：《中国实验血液学杂志》2002年第4期277-280,共4页Journal of Experimental Hematology

基　　金：国家自然基金;海外青年学者合作研究基金 编号 39928010

摘　　要：为了解作用于早期造血的细胞因子受体Flt3和c-kit在脐血CD34^+造血干/祖细胞中的表达及功能,从蛋白和基因水平对其进行了研究。采用常规双色直接免疫荧光标记法,使用流式细胞术测定新鲜分离的和体外不同培养时间的脐血CD34^+造血干/祖细胞中Flt3和c-kit蛋白水平的表达,用RT-PCR法测定Flt3和c-kit的mRNA水平表达,并在体外对受体功能进行了探讨。研究结果显示,新鲜脐血CD34^+细胞中(68.8±15.4)％为Flt3^+,(50.6±12.7)％为c-kit^+,随着体外培养时间的延长,二者表达逐渐下降。结论:脐血CD34^+造血干/祖细胞在体外液体培养条件下Flt3和c-kit阳性表达可维持2-3周,其配体FL和SCF在培养的第1周内对细胞扩增效果最显著。To investigate the expression and function of the receptors of early-acting cytokines on cord blood CD34 + hematopoietic stem/proginetor cells,the studies of Flt3 and c-kit were undertaken at the gene and protein levels.Fresh and cultured cord blood CD34 + stem/proginetor cells were analyzed by flow cytometric two-color direct immunoflurescence methods and RT-PCR,while function of receptors was studied in vitro.It was found that (68.8 + 15.4) % of CD34 + cells expressed Flt3,(50.6+12.7)% of CD34+ cells expressed c-kit,the proportion of CD34 + cells expressing Flt3 and c-kit decreased as in vitro culture time extended.It was concluded that cord blood CD34+ stem/proginetor cells are capable of expressing Flt3 and c-kit for as long as 2-3 weeks in liquid medium,during the first week of culture,SCF and FL enhanced the generation of cells and progenitors notably.

关 键 词：脐血 CD34^+细胞 造血干/祖细胞 FLT3 C-KIT 细胞分子 受体-配体网络系统 直接免疫荧光标记法 流式细胞术 

分 类 号：R331.14[医药卫生—人体生理学]