多发性骨髓瘤血管新生的体外研究  

Study on Angiogenesis of Multiple Myeloma in Vitro

作　　者：陈文明[1] 吴垠[1] 朱嘉芷[1] Jeannette Soria Massoud Mirshahi 

机构地区：[1]首都医科大学附属北京朝阳医院血液科,北京100020 [2]巴黎Hotel-Dieu医院

出　　处：《中国实验血液学杂志》2002年第4期310-314,共5页Journal of Experimental Hematology

基　　金：北京市科技新星项目部分资助项目 编号 954812300

摘　　要：探讨多发性骨髓瘤细胞系培养上清液对人骨髓来源内皮细胞系(HBMEC)的增殖、迁移及血管新生的作用。用含2％胎牛血清(FBS)的RPMI 1640培养液培养骨髓瘤细胞系获得上清液,将此培养液培养HBMEC,研究其时HBMEC的增殖及迁移的影响,并将此液放入含有携带HBMEC微珠的三维纤维蛋白中,研究其对血管新生的影响。结果表明,与含2％FBS的RPMI 1640培养液比较,骨髓瘤细胞系培养液对HBMEC的增殖有明显促进作用(P<0.001),并促进HBMEC的迁移,诱导毛细血管管腔的形成(长度及宽度)(P<0.001)。结论:骨髓瘤细胞系可能通过分泌某些细胞因子促进HBMEC增殖、迁移及血管新生。Angiogenesis is a necessary step in tumor progression,and it correlates an unfavorable prognosis.In multiple myeloma,bone marrow microvessel density and angiogenesis grading correlated with plasma cell labeling index and are poor survival predictors,but the study of myeloma's angiogenesis is very rare.This article was to study the effect of multiple myeloma cell lines conditioned media on the proliferation,migration and angiogenesis of human bone marrow endothelial cells (HBMEC).The multiple myeloma cell lines conditioned media were obtained by using RPMI 1640 media containing 2% fetal bovine serum (FBS) to cultivate myeloma cell lines for 18 houres.Proliferation and migration of HBMEC were detected by using those media to cultivate HBMEC.Capillary tube formation was performed by using microcarriers cytodex-3 covered with HBMEC in three-dimensional fibrin matrices.The results showed that myeloma conditioned media induced HBMEC's proliferation and migration ( P < 0.001),and those media induced capillary tube formation (length and width) of HBMEC ( P < 0.001).It was concluded that myeloma cell lines induce HBMEC's proliferation,migration,and capillary tube formation by secreting several cytokines.

关 键 词：多发性骨髓瘤 多发性骨髓瘤细胞系 骨髓来源内皮细胞系 血管新生 MM 

分 类 号：R733.3[医药卫生—肿瘤]