乳腺原发癌和相应淋巴结转移癌干细胞Wnt、Notch信号通路相关分子的比较  

Comparative study on Wnt,Notch signaling pathway molecules in primary breast cancer and matched lymph nodes metastasis stem cells

作　　者：曾令瑞 韩玉贞[2] 马英路[3] 李文星[2] 

机构地区：[1]滨州医学院附属医院病理科,滨州256603 [2]滨州医学院病理学教研室,滨州256603 [3]滨州医学院附属医院甲乳外科,滨州256603

出　　处：《临床与实验病理学杂志》2014年第9期963-966,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：山东省自然科学基金(ZR2010HM096)

摘　　要：目的探讨乳腺原发癌和相应淋巴结转移癌干细胞Wnt、Notch信号通路分子β-catenin、Cyclin D1和Notch1 mRNA表达及意义。方法选取乳腺浸润性导管癌30例,且均伴有淋巴结转移,制备乳腺癌单细胞悬液,通过免疫磁珠分选技术分别提取乳腺原发癌和相应淋巴结转移癌干细胞,采用实时荧光定量PCR技术检测两种癌干细胞Wnt、Notch信号通路关键分子β-catenin、Cyclin D1和Notch1 mRNA的表达。结果乳腺原发癌干细胞和相应淋巴结转移癌干细胞中β-catenin mRNA的表达差异无统计学意义(P>0.05);淋巴结转移癌干细胞中Cyclin D1和Notch1 mRNA的表达显著高于乳腺原发癌干细胞(P<0.01)。结论与乳腺原发癌干细胞相比,淋巴结转移癌干细胞中Cyclin D1和Notch1处于更高的活化状态,具有更高的侵袭和转移能力,有可能成为乳腺癌的新治疗靶点。Purpose To investigate the gene expression and significance of β-catenin,Cyclin D1 and Notch1 in primary breast cancer stem cells（ BCSC） and matched lymph node metastasis stem cells. Methods 30 cases of breast invasive ductal carcinoma and matched metastasis lymph nodes were made into single cell suspensions,then BCSC were separated from them by immunomagnetic sorting. β-catenin,Cyclin D1 and Notch1 gene expressions of Wnt,Notch signaling pathway were detected by real time PCR. Results The expression of β-catenin in primary BCSC and matched lymph nodes metastasis stem cells had statistically no differences（ P〉0. 05）,while the expression of Cyclin D1 and Notch1 in matched lymph nodes metastasis stem cells were significantly higher than the expression in primary BCSC（ P〈 0. 01,respectively）. Conclusion Compared with the primary cancer stem cells,Cyclin D1 and Notch1 activation in metastasis cancer stem cells are in higher level,which leades to a higher capability of invasion and metastasis,which may be a new therapeutic target.

关 键 词：乳腺肿瘤 癌干细胞 WNT信号通路 NOTCH信号通路 实时荧光定量PCR 

分 类 号：R737.9[医药卫生—肿瘤]