高效液相色谱法测定疲王按摩乳中阿魏酸的含量  

The Content Determination of Ferulic Acid in Piwang Massage Lotion by High Performance Liquid Chromatography

作　　者：涂禾[1] 梁栗[2] 侯世祥[2] 冯光富[1] 

机构地区：[1]四川省骨科医院药剂科,成都610041 [2]四川大学华西药学院

出　　处：《华西医学》2014年第9期1697-1700,共4页West China Medical Journal

基　　金：国家"十五"科技攻关项目(2002BA904B03)~~

摘　　要：目的建立疲王按摩乳中阿魏酸的含量测定方法。方法采用高效液相色谱法，色谱柱为Diamonsil C18（150mm×4．6mm，5μm，北京迪马科技有限公司）；流动相为甲醇．2％冰醋酸（32：68）；柱温为30℃；检测波长为320nm。结果阿魏酸在4．55～91．00μg／mL范围内呈良好的线性关系（R2=0．9998），其平均加样回收率分别为98．78％（相对标准偏差为2．668％）。结论该测定方法简便、快速、重现性好，可作为疲王按摩乳的质量控制方法。Objective To establish a method for content determination of ferulic acid in Piwang massage lotion. Methods High performance liquid chromatography was used. The analysis was carried on Diamonsil C18 （150 mm× 4.6 mm, 5 μm, Dimma Science and Technology Co. Ltd.） column with mobile phase consisted of methanol-2% acetic acid （32 ： 68）. The column temperature was 30 ℃ . The detection wavelength was 320 nm. Results The responses of ferulic acid liner in range of 4.55-91.00 μg/mL （R2=0.999 9）. The average recovery of ferulic acid was 98.78 % （relative standard deviation is 2.668 %）. Conclusions The method is simple, rapid and good repeatability. It can be used for quality control of Piwang massage lotion.

关 键 词：疲王按摩乳 高效液相色谱法 阿魏酸 含量测定 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]