人牙周膜干细胞条件培养液对舌癌Tca-8113细胞增殖及迁移能力的影响  

Effects of human periodontal ligament stem cells-conditioned medium on proliferation and migration of tongue carcinoma cell line Tca-8113

作　　者：王家奎[1] 高维岳[1] 秦子顺[1] 程文晓[1] 余占海[1] 殷丽华[1] 

机构地区：[1]兰州大学口腔医学院修复科,甘肃兰州730000

出　　处：《肿瘤》2014年第9期790-794,816,共6页Tumor

基　　金：国家自然科学基金青年科学基金项目(编号:81102712);中央高校基本科研业务费专项资金项目(编号:861500)

摘　　要：目的 :探讨人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)条件培养液(conditioned medium,CM)对舌癌Tca-8113细胞增殖及迁移能力的影响。方法 :从健康成人牙周膜组织中分离并培养hPDLSCs。第3代hPDLSCs融合达80%时更换为无血清培养液,继续培养24 h后收集上清液,即获得hPDLSCs-CM。舌癌Tca-8113细胞分别在常规培养液(对照组)和含50%hPDLSCs-CM的培养液(CM处理组)中培养,然后采用MTT法检测细胞增殖活性,FCM法分析细胞周期变化,Transwell迁移实验检测细胞迁移能力的变化。结果 :分离培养的hPDLSCs表达干细胞标志蛋白Stro-1和CD146,且具有明显的克隆形成及多向分化能力。将hPDLSCs-CM与舌癌Tca-8113细胞共培养至第3、5和7天时,CM处理组的细胞增殖活性均高于未处理对照组(P<0.01)。培养至第4天时,CM处理组的细胞增殖指数(proliferative index,PI)较对照组明显升高(P<0.01)。培养24 h后,CM处理组穿膜细胞数明显高于对照组(P<0.01)。结论 :hPDLSCs-CM可以促进舌癌Tca-8113细胞增殖,并增强该细胞的迁移能力。Objective： To investigate the effects of human periodontal ligament stem cells （hPDLSCs）- conditioned medium （CM） on cell proliferation and migration of tongue carcinoma cell line Tca-8113. Methods： hPDLSCs were isolated from healthy adults and culture-expanded. When the cells of passage 3 reached 80% confluence, the medium was changed to fresh medium not containing fetal bovine serum. After 24 h incubation, the supernatant was collected and named as hPDLSCs-CM. Then the Tca-8113 cells were incubated in fresh medium alone （as the control group） or in the medium supplemented with 50% hPDLSCs-CM （as the CM group）. The cell proliferative activity was measured by MTT assay, the cell cycle was analyzed by flow cytometry （FCM）, and the cell migration ability was measured by Transwell chamber assay. Results： The hPDLSCs, which were isolated and culture-expanded, expressed mesenchymal stem cell-related antigens Stro-1 and CD146, and had the abilities of colony-formation and multilineage differentiation. The proliferation abilities of Tca-8113 cells after co-cultrue with hPDLSCs-CM for 3, 5 and 7 d were significantly higher than those of the control group （P 〈 0.01）. After being co-cultured for 4 d, the cell proliferation index （PI） of the CM group was significantly higher than that of the control group （P 〈 0.01）. After being co-cultured for 24 h, the number of the cells passing across the membrane in the CM group was significantly much more than that in the control group （P 〈 0.01）. Conclusion： hPDLSCs-CM can promote the proliferation and improve the migration ability of Tca-811 3 cells.

关 键 词：舌肿瘤 牙周膜 间质干细胞 培养液 条件性 细胞增殖 细胞迁移分析 

分 类 号：R739.86[医药卫生—肿瘤]