不同分期溃疡性结肠炎患者黏膜相关菌群的变化及意义  

Changes of mucosa associated bacterial flora in patients with ulcerative colitis at different stages and its significance

作　　者：褚源[1] 王巧民[1] 

机构地区：[1]安徽医科大学附属省立医院消化内科,合肥230001

出　　处：《安徽医科大学学报》2014年第10期1444-1447,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生厅医学科研课题(编号:13zc031)

摘　　要：目的研究活动期、缓解期溃疡性结肠炎(UC)患者肠黏膜标本中梭菌属、肠球菌属、乳酸杆菌属、双歧杆菌属及拟杆菌属、大肠杆菌属这6种菌群含量,探讨肠道膜菌群改变在UC发病中的可能作用及意义。方法收集35例UC患者(活动期组20例、缓解期组15例)及20例健康体检者(对照组)乙状结肠黏膜活检组织,提取标本细菌DNA,根据细菌的16SrDNA序列设计特异性引物,应用实时荧光定量PCR法测定不同细菌的数量。结果与对照组相比,活动期组的肠黏膜标本中大肠杆菌含量增加,双歧杆菌属、拟杆菌属、乳酸杆菌属、梭菌属含量减少(P<0.05),但肠球菌属含量无明显变化;与对照组比较,缓解期组拟杆菌属及双歧杆菌属减少(P<0.05),大肠杆菌属、乳酸杆菌属、梭菌属、肠球菌属含量无明显变化。活动期组患者双歧杆菌/大肠杆菌(B/E)值<1,较对照组降低,而缓解期组与对照组B/E值均>1,且两者间差异无统计学意义。结论 UC患者结肠黏膜存在明显的菌群变化,以活动期UC更明显,提示肠道菌群改变与UC发生发展密切相关。Objective Sigmoid mucosa specimens of the patients with ulcerative colitis （ UC ） at active stage and remission stage were respectively detected by real-time PCR for the contents of the six kinds of bacterial floras inclu-ding fusobacterium, enterococcus, lactobacillus, bifidobacterium, bacteroides, and escherichia coli. So the possi-ble roles and significance of the changes of intestinal mucosa associated bacterial flora in the pathogenesis of UC were discussed. Methods Sigmoid biopsy tissues were collected from 35 UC patients （ 20 cases were activities group while 15 cases were remission group） and 20 healthy cases（ control group） . Specific primers were set accord-ing to the bacterial 16 SrDNA sequences. Bacterial DNA of the intestinal mucosa specimens was extracted, and re-al-time PCR was used to detect the numbers of different bacterial colonies. Results In sigmoid mucosa specimens of the UC group at activities group, escherichia coli colony was increased, while bifidobacterium, bacteroides, lac-tobacillus and fusobacterium, were reduced compared to the control group（P〈0.05）. But for enterococcus, there was no significant change（P〉0.05）. And in remission group, bacteroides and bifidobacterium were reduced com-pared with the control group（P〈0.05）, while no significant changes were found in escherichia coli, lactobacillus, fusobacterium and enterococcus（ P 〉0.05 ） . The ration of bifidobacterium to escherichia coli （ B/E ） in UC pa-tients at active stage was less than 1, which was lower than the control group. While B/E values in UC patients at remission stage and the control group were both larger than 1 , with no statistically significant difference between them. Conclusion There were obvious changes of intestinal bacterial flora in UC patients, and the change is more obvious in the UC patients at active stage, showing that there is a close relationship between intestinal mucosa asso-ciated bacterial flora and the development of UC.

关 键 词：溃疡性结肠炎 黏膜相关菌群 

分 类 号：R574[医药卫生—消化系统]