血尿酸对大鼠阿尔茨海默病生物标记物表达的影响  

The effects of serum uric acid on expression of APP and BACE1 in rats

作　　者：王君霞[1] 刘庆新[1] 肖新兴 

机构地区：[1]滨州医学院附属医院神经内科,滨州256600

出　　处：《中国神经精神疾病杂志》2014年第8期479-482,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：山东省高等学校科技计划项目(编号:J12LL04)

摘　　要：目的观察不同血尿酸水平对大鼠阿尔茨海默病生物标记物β-淀粉样肽前体蛋白(amyloid recurosr protein,APP)及β分泌酶-1(beta-site amyloid precursor protein cleaving enzyme-1,BACE1)表达的影响。方法采用腹腔注射氧嗪酸钾盐(oxonic acid Potassium salt,OAPS)的方法建立不同水平高尿酸血症(hyperuricemia,HUA)大鼠模型,HE染色观察大鼠海马区形态学变化;Western blot检测海马区APP及BACE1的表达,并进行统计学分析。结果与正常对照组比较,OAPS处理组血尿酸水平明显升高(P<0.01),海马区APP及BACE1的表达明显上调(P<0.001),与低剂量组比较,中、高剂量组血尿酸水平升高(P<0.05),海马区APP及BACE1表达减少(P<0.05),与中剂量组比较,高剂量组血尿酸水平增高(P<0.01),高剂量组的大鼠海马组织APP的表达减少(P<0.05),BACE1表达无统计学差异性(P>0.05)。结论高于正常水平的血尿酸可能为AD发生或发展的危险因素,其水平越高对AD危险性越低。Objective To observe the effects of the different serum uric acid levels on expression of Alzheimer’s disease biomarkers （APP and BACE1） in rats. Methods Intraperitoneal injection of oxygen of oxazine acid potassium was used to produce HUA models in rats. H＆amp;E staining was used to detect the morphological changes of the hippocampus. Western blot was used to detect the protein levels of APP and BACE1 of the hippocampus. Results Compared with nor-mal control group, the serum uric acid and the protein levels of APP and BACE1 in the hippocampus was obviously in-creased at OAPS treatment group （P〈0.01）. Compared with low dose OAPS treatment group, the serum uric acid level was significantly increased whereas the protein levels of APP and BACE1 in the hippocampus were significantly decreased at the middle and high dose group （P〈0.01）. Compared with middle dose group, the serum uric acid level was increased at high dose group （P〈0.05） and the expression of APP were decreased in the hippocampus rats but the expression of BACE1 remained unchanged （P〉0.05）. Conclusion The higher level of serum uric acid may be a protective factor of AD. The higher serum uric acid levels, the lower the risk of AD.

关 键 词：高尿酸血症 β-淀粉样肽前体蛋白 β分泌酶-1 

分 类 号：R749.16[医药卫生—神经病学与精神病学]