miR-19在肝细胞肝癌中的表达及其临床意义  

MiR-19 expression in hepatocellular carcinoma and its clinical significance

作　　者：郭跃虎[1] 刘建武[1] 王波[1] 赵巍[1] 何明艳[1] 

机构地区：[1]武警山西总队医院普通外科,山西太原030006

出　　处：《中国普通外科杂志》2014年第9期1217-1221,共5页China Journal of General Surgery

摘　　要：目的:探讨肝细胞肝癌(HCC)组织中miR-19的表达情况及其临床意义。方法:用qRT-PCR的方法检测51例HCC组织及其癌旁组织以及10例正常肝组织中miR-19的表达;分析了miR-19表达与HCC患者临床病理因素及预后的关系。结果:miR-19的表达量在正常肝组织、癌旁组织、HCC组织中依次增高,差异均有统计学意义(均P<0.05)。miR-19表达与HCC患者的肿瘤大小(P=0.029)、转移(P=0.011)、静脉侵犯(P=0.002)及AJCC分期(P=0.008)有关,而与年龄、性别、肿瘤分化、卫星灶、肿瘤数量及AFP无相关(均P>0.05)。miR-19高表达的患者5年生存率明显低于低表达的患者(P<0.05)。miR-19高表达以及转移、静脉侵犯均为影响HCC患者生存的独立风险因素(均P<0.05)。结论:miR-19在HCC组织中表达上调,并可能与HCC的增殖、转移有关;miR-19高表达提示预后不良。Objective： To investigate the miR-19 expression in hepatocellular carcinoma （HCC） tissue and its clinical significance. Methods： The miR-19 expression in 51 pairs of HCC tissues along with their matched adjacent tissues, and 10 samples of normal liver tissues were detected by qRT-PCR methods. The relations ofmiR-19 expression with the clinicopathologic factors and prognosis of HCC patients were analyzed. Results： The miR-19 expression level was increased in ascending order in normal liver tissue, tumor-adjacent tissue and HCC tissue, and the differences had statistical significance （all P〈0.05）. The miR-19 expression was significantly related to tumor size （P=0.02）, metastasis （P=0.011）, venous invasion （P=0.002） and AJCC stage （P=0.008） of the HCC patients, but irrelevant to their age, sex, tumor differentiation, satellite lesions, tumor number and AFP level （all P〉0.05）. The 5-year survival rate in patients with high miR-19 expression was significantly lower than that in those with low miR-19 expression （P〈0.05）. High miR-19 expression togetherwith metastasis and venous invasion are risk factors affecting the survival of HCC patients （all P〈0.05）. Conclusion： The miR-19 expression is up-regulated in HCC tissue, which probably may be associated with the proliferation and metastasis of HCC, and high miR-19 expression may predict an Unfavorable outcome.

关 键 词：癌 肝细胞 微RNAS MicroRNA-19 预后 

分 类 号：R735.7[医药卫生—肿瘤]