16S rRNA基因测序技术在肝脓疡细菌鉴定中的作用(英文)  

Usefulness of 16S rRNA Gene Sequencing for Identification of Bacteria from Pyogenic Liver Abscess

作　　者：宋伦圭 Yun Gyu Song Sang Gun Shim Kwang Min Kim Dae Soo Kim Sang Haeng Choi Jae Young Song Kon Ho Lee Hyung-Lyun Kang Seung-Chul Baik Woo-Kon Lee Myung Je Cho Kwang Ho Rhee Dong Hae Lee 

机构地区：[1]庆尚南道昌原市三星昌原医院,成均馆大学医院 [2]Department of Radiology, Samsung Changwon Hospital,Sungkyunkwan University School of Medicine, Changwon, Korea [3]Department of Internal Medicine, Samsung Changwon Hospital,Sungkyunkwan University School of Medicine, Changwon, Korea [4]Human Derived Material Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea [5]Unitech Science Co., Ltd., Daejeon, Korea [6]Department of Microbiology, Gyeongsang National University College of Medicine, Jinju, Korea

出　　处：《介入放射学杂志》2014年第10期906-912,共7页Journal of Interventional Radiology

摘　　要：目的评价16S核糖体RNA(rRNA)基因测序在肝脓疡中细菌鉴定中的应用价值。方法2012年1月—2013年12月间共20例肝脓疡行经皮置管引流的患者,分别行脓液培养,血培养和16S rRNA基因测序。利用454 GS Junior System对脓液基因组DNA行PCR和16S rRNA基因测序。脓液培养,血液培养和16S rRNA基因测序结果进行分别评价。结果脓液和血液培养阳性的患者分别是9例(45%)和4例(20%)。16S rRNA基因测序细菌鉴定率为90%,明显高于传统的培养方法。结论 16S rRNA基因测序方法较传统的培养方法能更准确和有效对肝脓疡进行细菌鉴定。Background/Aims To evaluate the usefulness of 16S ribosomal RNA （rRNA） gene sequencing for an accurate and better identification of bacteria from pyogenic liver abscess （PLA）. Methodology 20 patients with PLA were included who underwent percutaneous catheter drainage, abscess culture, blood culture and 16S rRNA gene sequencing for isolates from January 2012 to December 2013. Genomic DNAs of abscess fluids were subjected to PCR and sequencing of 16S rRNA gene by on a 454 GS Junior System. The results were evaluated between abscess cultures, blood and 16S rRNA gene sequencing for isolates. Results Abscess and blood cultures were positive in 9 （45%） and 4 （20%） patients, respectively. The 16S rRNA gene sequencing showed with 90% identification of bacteria a significantly greater identification than conventional cultured methods. Conclusion This study showed a greater usefulness of 16S rRNA gene sequencing than conventional cultured methods for accurate and better identification of bacteria from PLA.

关 键 词：肝脓疡 脓肿培养 宏基因组学 16S rRNA基因测序 

分 类 号：R575.4[医药卫生—消化系统]