浆样树突细胞在感染诱发白血病自发缓解中的免疫作用  

Immuno-effect of plasmacytoid dendritic cells on bacteria infection induced spontaneous remission of leukemia

作　　者：李莉娟[1] 张连生[1] 柴晔[1] 曾鹏云[1] 吴重阳[1] 岳玲玲[1] 白俊[1] 郝正栋[1] 胡婉丽[1] 陈慧玲[1] 郭晓嘉[1] 

机构地区：[1]兰州大学第二医院,730030

出　　处：《中华血液学杂志》2014年第10期880-884,共5页Chinese Journal of Hematology

基　　金：国家自然科学基金（81372533）;甘肃省科技重大专项（1302FKDA029）

摘　　要：目的 探讨浆样树突细胞（pDC）在感染诱发白血病自发缓解（SR）中的免疫作用.方法 分离白血病SR患者及健康人外周血单个核细胞,应用免疫磁珠阴性选择及流式细胞阳性选择双次分选法,获得纯化的人pDC和髓样树突细胞（mDC）.将pDC分别与不同细菌共培养,观察其活化情况,MTT法检测T细胞增殖能力,ELISA法检测细胞因子（IFN-α、IL-12、IFN-γ、IL-2、IL-4、IL-10）水平的变化.结果 金黄色葡萄球菌、铜绿假单胞菌能刺激白血病SR患者pDC分泌大量IFN-α,孵育48 h,pDC上清液中IFN-α水平分别为（15.34±2.91）ng/ml和（10.38±1.41）ng/ml,较阴性对照组（脂多糖＋pDC）[（1.36±0.13）ng/ml]明显升高（P值均＜0.01）.活化的pDC能促使初始CD4＋T细胞向Th1细胞分化[培养上清IFN-γ水平分别为（2.16±0.37）ng/ml和（2.73±1.11）ng/ml,平行对照组（脂多糖活化mDC＋T细胞）为（2.55±0.23）ng/ml,P＞0.05],并使T细胞增殖能力增强,细胞增殖指数分别为4.36及4.05,高于非活化组的1.23及0.13（P＜0.01）.金黄色葡萄球菌、铜绿假单胞菌对健康人来源pDC具有同样的活化能力.结论 金黄色葡萄球菌、铜绿假单胞菌活化的pDC在感染诱导的白血病SR中可能发挥重要作用.Objective To explore the immuno-effect of plasmacytoid dendritic cells （pDC） on bacteria infection induced spontaneous remission （SR） of leukemia. Methods Both pDC and myeloid dendritic cells （mDC） were isolated and purified from leukemic patient with SR and healthy donor by combination of immunomagnetic beads and flow cytometry, pDC were cultured in RPMI1640 medium and stimulated with different bacteria. The T cells proliferation was detected by MTT, and cytokine production by ELISA kits. Results The human bacterial pathogen Staphylococcus aureus and Pseudomonas aeruginosa stimulation for 48 h resulted in the maturation of pDC with production of high quantity of IFN-ct at （15.34＋2.91） ng/ml and （10.38~ 1.41） ng/ml, respectively, comparing with that of negative group at （1.36~0.13） ng/ml （P 〈 0.01 ）. Activated pDC could promote the differentiation of naive CD4＋T cells to Thl cells with secretion of IFN-7 at （2.16±0.37） ng/ml and （2.73± 1.11） ng/ml, respectively, comparing with that of positive control at （2.55 ±0.23） ng/ml （P〉0.05）. Activated pDC showed higher T cell stimulatory capacities [proliferation index （PI） was 4.36 and 4.05, respectively] than that of non-activated pDC （PI was 1.23 and 0.13, respectively） （P〈0.01）. Conclusion Staphylococcus aureus and Pseudomonas aeruginosa activated pDC may play a key role in SR of leukemia following severe infections.

关 键 词：白血病 肿瘤消退 自行性 树突细胞 感染 

分 类 号：R733.7[医药卫生—肿瘤]