肺癌患者外周血调节性T细胞的变化及其与临床病理因素的关系  

Changes of regulatory T cells in peripheral blood samples from patients with lung cancer and their correlation with clinicopathologic features of lung cancer

作　　者：吴杰[1] 陈文虎[1] 张钧[2] 骆骥才[3] 张剑英[1] 张毅敏[1] 潘志文[1] 单绿虎[1] 熊娟[1] 

机构地区：[1]浙江省肿瘤医院检验科,杭州310022 [2]浙江大学医学院附属邵逸夫医院检验科 [3]浙江大学医学院附属第一医院检验科

出　　处：《中华微生物学和免疫学杂志》2014年第9期687-690,共4页Chinese Journal of Microbiology and Immunology

基　　金：浙江省重点科技计划项目（2007C23008）

摘　　要：目的：探讨肺癌患者外周血CD4＋CD25＋CD127low调节性T细胞（Treg）水平变化的意义及其与临床病理因素的关系。方法采用流式细胞术测定160例肺癌患者组和60例健康对照组外周血中CD4＋、CD8＋、自然杀伤细胞（NK）以及Treg细胞等不同淋巴细胞亚群的比例，比较两组间的差异，同时分析不同临床病理因素下Treg细胞的差异，选取其中60例患者比较术前和术后一个月Treg细胞的变化。结果肺癌组血浆CD4＋和NK细胞比例以及CD4＋/CD8＋比值均显著低于健康对照组，而Treg细胞显著高于健康对照组，且在不同的TNM分期中，Ⅲ、Ⅳ期组患者的Treg细胞比例要显著高于Ⅰ、Ⅱ期，差异有统计学意义（P均＜0．05）；手术后Treg细胞比例显著下降，差异亦有统计学意义（P＜0．01）。结论肺癌患者中Treg细胞抑制了正常的抗肿瘤功能，并与肺癌的分期和进展有关，在肺癌预后判断以及疗效监测中有重要意义。Objective To investigate the changes of CD 4^＋CD25^＋CD127^low regulatory T （ Treg ） cells in peripheral blood samples from patients with lung cancer and their correlation with clinicopathologic features of lung cancer .Methods Flow cytometry was used to measure the percentages of CD 4, CD8, nat-ural killer （ NK） and Treg cells in peripheral blood samples collected from 160 patients with lung cancer and 60 healthy subjects .The correlations between the levels of Treg cells and clinicopathologic features of lung cancer were analyzed .The percentages of Treg cells in 60 patients with lung cancer before and after surgery were compared .Results The percentages of CD 4＋and NK cells and the ratios of CD 4＋/CD8＋cells in pa-tients with lung cancer were significantly lower than those in healthy control , while the percentages of Treg cells in patients with lung cancer were decreased as compared with those in healthy subjects .The percenta-ges of Treg cells in patients with advanced cancer were significantly higher than those in patients at early stage （P〈0.05）, which dropped significantly after surgery （P〈0.01）.Conclusion The results of this study indicated that Treg cells in patients with lung cancer might inhibit antitumor immune responses and correlate with the progression of cancer .It would be worthwhile to check Treg cells in patients with lung cancer for monitoring the prognosis and treatment effects .

关 键 词：调节性T细胞 肺癌 流式细胞术 CD4^+CD25^+CDl27^low 

分 类 号：R734.2[医药卫生—肿瘤]