核心组蛋白H2A与乳腺癌预后相关因素的生存分析  

Survival Analysis of Prognostic Factors A between Core Histone H2A and Breast Cancer

作　　者：王蓓莉[1] 应明真[2] 蒋马伟[1] 陈光榆[1] 王雅杰[2] 

机构地区：[1]上海交通大学医学院附属新华医院肿瘤科,200092 [2]第二军医大学附属长海医院肿瘤科,上海200433

出　　处：《医学研究杂志》2014年第9期17-21,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(81072175;81102010;81372854);上海市科委基础研究重点项目(13NM1401504);上海市重点学科建设项目(B905)

摘　　要：目的 本研究探讨核心组蛋白（core histone H2A,H2A）在乳腺癌组织中的表达及相关乳腺癌病例术后生存数据预后因素的分析。方法 应用免疫组化Envision方法,检测97例乳腺癌组织中H2A的表达情况,回顾性分析患者的临床资料。分别采用Kaplan-Meier法进行生存分析,log rank检验进行生存率比较。选择单因素分析（log rank检验）中有统计意义的变量,采用Cox比例风险回归模型分析患者术后生存数据,解析影响预后的多种因素,Spearmen相关分析解析其和H2A表达强度之间的相关性。结果 生存分析显示,H2A的表达强度对预后无明显影响（P＞0.05）。但值得注意的是,H2A的表达生存分析在30个月左右后出现“有差异”的趋势。对TNM Ⅰ～Ⅱ期和Ⅲ～Ⅳ期的病例分别行H2A表达的亚组分析显示,两亚组中死亡病例均为H2A阴性患者,并且在TNM分期恶性程度更高的Ⅲ～Ⅳ期中,所有病例均为H2A阴性患者,无H2A阳性患者。Spearmen相关分析结果显示,H2A的表达强度与TNM分期呈负相关（r=-0.242,P=0.026）。结论 H2A的低表达则常常预示着TNM分期晚,更趋于恶性,并且在TNMⅢ～Ⅳ期中H2A的低表达预示着生存率更低。H2A蛋白的表达可能成为对乳腺癌恶性潜能甄别的指标。Objective To evaluate the expression and prognostic factors analysis of H2A in breast cancertissue.Methods The expression of H2A was detected by immuneohistochemical method of Envision.A clinical data of ninety-seven cases of breast cancer patients were respectively analysed.First,survivalanalysis was calculated by Kaplan-Meier method,and survival rate was compared by log rank test.Second,we selected statistically significant variables in the single factor analysis （the log rank test）.The postoperative survival data and prognostic factors were analyzed by Cox proportional hazards regression model.The correlation between H2A and clinical pathological indicators was analyzed by spearmen correlation analysis.Results Survival analysis showed the expression intensity of H2A had no obvious effect on the prognosis （P ＞ 0.05）.But,it was notable that the expression intensity of H2A in survival analysis appeared the tendency of ＂difference＂ after 30 months.The cases of TNM stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ were separated to two subgroups and analyed respectively to survey the expression of H2A.The deaths in the two subgroups were both H2A negative.And in the subgroup of TNM stage Ⅲ -Ⅳ which was more malignant,all cases were H2A negative while no H2A positive patients.Spearmen correlation analysis showed the expression intensity of H2A was negatively related with TNM staging（r =-0.242,P =0.026）.Conclusion The lower expression of H2A is often predicting late TNM staging,and tends to be more malignant.And furthermore,in TNM stage Ⅲ-Ⅳ,the lower expression of H2A indicates the poor survival rate.The expression of H2A may become a screened indicator of breast malignant potential.

关 键 词：乳腺癌 免疫组化 H2A 预后 

分 类 号：R737[医药卫生—肿瘤]