肌上皮细胞在腮腺再生过程中的变化  

Changes of myoepithelial cells during regeneration of parotid gland

作　　者：毛玉龙[1] 张伟伟[2] 左金华[1] 

机构地区：[1]滨州医学院附属医院口腔颌面外科,滨州256603 [2]滨州医学院附属医院口腔正畸科,滨州256603

出　　处：《华西口腔医学杂志》2014年第5期446-449,共4页West China Journal of Stomatology

基　　金：山东省医药卫生科技发展计划基金资助项目(2011HW004)

摘　　要：目的研究腮腺再生过程中的肌上皮细胞(MEC)数量及分布的变化。方法 54只Wistar大鼠分为8个实验组和1个正常对照组,每组6只。实验组结扎大鼠右侧腮腺主导管,结扎后第14天使其再通,分别于再通后第0、1、3、5、7、10、14、21天获取腮腺组织标本,应用苏木精-伊红染色观察再生腺体的组织学变化,并采用免疫组织化学染色法定量分析MEC在腮腺再生不同时间点的数量及分布情况,并与正常对照组作比较。结果腮腺组织于主导管结扎后第14天明显萎缩,多数腺泡细胞消失,导管样结构明显增多;而MEC数量明显增加,主要分布在导管样结构周围。导管再通后,从第3天开始,腺泡细胞明显增加,导管样结构明显减少,同时MEC数量减少,主要分布在新生腺泡及导管样结构周围,导管再通第3、5天时MEC数量下降最为明显;再通14 d后,腺体结构和MEC的数量及分布基本恢复正常,与正常对照组无明显差异。结论 MEC的数量和分布在腮腺萎缩后再生的第14天可基本恢复正常,腮腺的再生主要发生在主导管再通后的5 d内。Objective To investigate the changes on the number and distribution of myoepithelial cells（MECs） during parotid gland regeneration. Methods A total of 54 Wistar rats were divided into eight experimental groups and one normal control group, with six rats in each group. The right parotid ducts of the rats in the experimental groups were ligated for 14 days and then reopened. The parotid tissue specimens were harvested at days 0, 1, 3, 5, 7, 10, 14, and 21. The histological changes of the regenerating gland were examined using hematoxylin–eosin staining. Immunohistochemical labeling was also performed to investigate the changes in the number and distribution of MECs at different time points of parotid regeneration. Both the histological and immunohistochemical changes observed in the experimental groups were compared with those in the normal control group. Results The parotid gland showed marked atrophy 14 days after ligation. Most acinar cells disappeared, but the number of duct-like structures obviously increased. MECs apparently increased in number and were mainly located at the periphery of the duct-like structures. Three days after duct reopening, the number of acinar cells significantly increased and the duct-like structures significantly reduced. Meanwhile, MECs also decreased in number and were mainly located at the periphery of the newly formed acini and duct-like structures. The number of MECs noticeably decreased 3 and 5 days after duct reopening. At 14 days after duct reopening, the glandular structures and the number and distribution of MECs returned to normal compared with those in the normal control group. Conclusion The number and distribution of MECs returned to normal condition after parotid gland atrophy. Parotid regeneration mainly occurred within 5 days after duct reopening.

关 键 词：肌上皮细胞 腮腺 萎缩 再生 

分 类 号：R739.8[医药卫生—肿瘤]