磷脂酶C-γ1_(tyr783)在下颌功能前伸大鼠髁突软骨后部表达变化的研究  

Changes in expression of phospholipase C-gamma1_(tyr783) in young rat condylar cartilage during functional mandibular protraction

作　　者：盛海莹 左艳萍[1] 陈潇[1] 苑学微[1] 

机构地区：[1]河北医科大学第一医院口腔正畸科,石家庄050031

出　　处：《华西口腔医学杂志》2014年第5期455-458,共4页West China Journal of Stomatology

摘　　要：目的研究大鼠下颌功能前伸后髁突软骨中磷脂酶C-γ1 tyr783(PLC-γ1 tyr783)表达及其变化规律,探讨PLC-γ1tyr783在功能矫治前伸大鼠下颌髁突软骨骨改建中的作用,为临床上骨骼矫形治疗提供实验依据。方法选取4周龄健康雄性Sprague-Dawley(SD)大鼠60只,适应性饲养1周后随机等量分为实验组和对照组,实验组每天24 h佩戴自制下颌前伸斜面导板矫治器,对照组不戴矫治器。2组大鼠分别于戴用矫治器1、3、7、14、21、28 d时各断颈处死5只,取双侧髁突,常规固定、脱钙、脱水、包埋。石蜡切片采用免疫组织化学方法结合图像分析检测PLC-γ1tyr783在髁突软骨中的表达分布及其变化规律。结果对照组PLC-γ1tyr783表达水平随着实验周期的推移逐渐减弱(P>0.05),实验组大鼠髁突软骨处PLC-γ1tyr783的表达在第14天时达到高峰,与对照组相比差异有统计学意义(P<0.01)。结论 PLC-γ1tyr783参与了下颌功能前伸大鼠髁突软骨的骨改建。Objective To investigate the changes in the expression of phospholipase C-gammaltyr783（PLC-γ1tyr783） in the condylar cartilage of a young rat during functional mandibular protraction. This work also explores the function of PLC-γ1tyr783in the rat mandibular condylar cartilage bone remodeling, which could provide experimental evidence for clinical bone orthopedic work. Methods A total of 60 four-week-old male Sprague–Dawley（SD） rats were used in this study. The rats were divided equally and randomly into experimental group and control group. The functional appliances that were fitted to the upper incisors of the animals in the experimental group were worn 24 h a day after the rats were fed for 7 d with homemade pellet feed. The animals in the experimental group, along with their matched controls, were sacrificed after 1, 3, 7, 14, 21, and 28 d. The bilateral condylar was fixed, decalcified, dehyded, and then conventional paraffin embedded. Immunohistochemistry of PLC- γ1tyr783 was applied to observe its express distribution and variation. Results The expression of PLC-γ1tyr783decreased gradually in the control group, which showed age-related changes（P〉0.05）. On the 14 th day, PLC-γ1tyr783 expression in the experimental group was significantly higher than that in the control group. PLC- γ1tyr783 expression began to appear statistically and significantly different between the two groups（P〈0.01）. Conclusion PLC-γ1tyr783 is involved in the bone remodeling process of the rat condylar cartilage after functional mandibular protraction.

关 键 词：下颌功能前伸 髁突软骨 磷脂酶C-Γ1 tyr783 骨改建 

分 类 号：R783.5[医药卫生—口腔医学]