丝/苏氨酸蛋白激酶/哺乳动物雷帕霉素靶蛋白/p70 S6K信号通路在口腔鳞癌中的表达及临床意义  

Expression and clinical significance of serine-threonine kinase/mammalian target of rapamycin/p70 S6K signal pathway in oral squamous cell carcinoma

作　　者：贾洁茹 梁爽[1] 刘济远[1] 赵君[1] 唐休发[1] 

机构地区：[1]口腔疾病研究国家重点实验室华西口腔医院头颈肿瘤外科(四川大学),成都610041

出　　处：《华西口腔医学杂志》2014年第5期504-508,共5页West China Journal of Stomatology

基　　金：四川省科技支撑计划基金资助项目(2011SZ0156)

摘　　要：目的探讨丝/苏氨酸蛋白激酶(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)/p70 S6K信号通路在口腔鳞癌组织中的表达情况,为口腔鳞癌的早期诊断和治疗提供参考。方法收集口腔鳞癌标本51例,癌旁黏膜组织10例,正常口腔黏膜10例。采用免疫组织化学SP法检测口腔鳞癌、癌旁黏膜组织及正常口腔黏膜中p-Akt、p-mTOR及p70S6K的表达情况,分析三者相互之间表达的相关性。结果 p-Akt、p-mTOR及p70 S6K在口腔鳞癌组中的表达显著高于正常口腔黏膜组和癌旁黏膜组的表达。p-Akt、p-mTOR及p70 S6K在口腔鳞癌的表达与患者的年龄、性别及临床分期无相关性,但与口腔鳞癌的分化程度及有无淋巴结转移有相关性。p-Akt、p-mTOR及p70 S6K在口腔鳞癌的表达相互之间具有较强的正相关关系。结论 Akt/mTOR/p70 S6K信号通路分子在口腔鳞癌中表达活跃,提示可能与口腔鳞癌的发生发展具有重要的相关性。Objective To investigate the expressions of serine-threonine kinase（Akt）/ mammalian target of rapamycin（mTOR）/p70 S6 K in oral squamous cell carcinoma（OSCC） and provide references for early diagnosis and prognosis evaluation of OSCC. Methods A total of 51 cases of OSCC, 10 cases of paracancerous mucosa, and 10 cases of normal oral mucosa were collected. The expressions of Akt/mTOR/p70 S6 K in these cases were detected using the SP method of immunohistochemistry. The correlation between their expressions in OSCC was also analyzed. Results The positive expressions of p-Akt, p-mTOR, and p70 S6 K in OSCC were significantly higher than those in normal oral mucosa and paracancerous mucosa. The expressions of p-Akt, p-mTOR, and p70 S6 K in OSCC were not correlated with age, gender, and clinical stage; by comparison, these expressions were correlated with lymph node metastasis and pathological grade. Strong positive correlations were also observed between the expressions of p-Akt, p-mTOR, and p70 S6 K in OSCC. Conclusion Akt/mTOR/p70 S6 K signaling molecules exhibit active expressions in OSCC and may be implicated in the occurrence and development of OSCC.

关 键 词：丝/苏氨酸蛋白激酶 哺乳动物雷帕霉素靶蛋白 P70 S6K 口腔鳞癌 免疫组织化学 

分 类 号：R739.8[医药卫生—肿瘤]