Time-effect Relationship of Extracts from Ginseng,Notoginseng and Chuanxiong on Vascular Endothelial Cells Senescence  

作　　者：王铭 雷燕 

机构地区：[1]The Cardiology Department of Chongqing Hospital of Traditional Chinese Medicine [2]Chongqing the First Hospital [3]The Experimental Research Center,China Academy of Chinese Medical Sciences

出　　处：《Chinese Journal of Integrative Medicine》2014年第10期758-763,共6页中国结合医学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China(No.30973828);Major State Basic Research Development Program of China(937 Program,No.2007CB507400);Self-Project Foundation from China Academy of Chinese Medical Sciences(No.Z02151)

摘　　要：Objective：To observe the time-effect relation of extracts from ginseng,notoginseng and chuanxiong on angiotensin II（AngⅡ）-induced senescence of vascular endothelial cells and explore the feature of Chinese medicine against vascular diseases.Methods：Human umbilical vein endothelial cells（HUVECs）cultured in vitro were stimulated with 10^-6 mol/L Ang Ⅱ to induce cell senescence,which were divided into 4groups,the blank control group,the Ang Ⅱ model group,the extracts group and the telmisartan group.The（J-gal was used to identify senescence of cells,the cell counting kit-8 method was applied to assess the cell viability,the cell function was examined with the level of endothelial nitric oxide synthase（eNOS） and the flow cytometry was used for analyzing the cell cycle changes.Results：Compared with the control cells,the cells positive for β-gal staining was significantly increased in the Ang Ⅱ model group,and showed cell cycle arrest at G0/G1 phase with decreased S and G2/M phase ceil percentage,eNOS expression and cell viability（P〈0.05）.The extracts and telmisartan treatment of Ang II-induced cells resulted in decreased β-gal positive cells with a reduction in G0/G1phase cells and an increasing in S,G2/M phase ceils and eNOS expression（P〈0.05）.At 24 h,the extracts were more effective than telmisartan（P〈0.05）;while telmisartan was more effective at 48 h（P〈0.05）.Conclusion：Extracts from ginseng,notoginseng and chuanxiong can delay Ang II-induced aging of HUVECs and may play an important role in early senescence.Objective：To observe the time-effect relation of extracts from ginseng,notoginseng and chuanxiong on angiotensin II（AngⅡ）-induced senescence of vascular endothelial cells and explore the feature of Chinese medicine against vascular diseases.Methods：Human umbilical vein endothelial cells（HUVECs）cultured in vitro were stimulated with 10^-6 mol/L Ang Ⅱ to induce cell senescence,which were divided into 4groups,the blank control group,the Ang Ⅱ model group,the extracts group and the telmisartan group.The（J-gal was used to identify senescence of cells,the cell counting kit-8 method was applied to assess the cell viability,the cell function was examined with the level of endothelial nitric oxide synthase（eNOS） and the flow cytometry was used for analyzing the cell cycle changes.Results：Compared with the control cells,the cells positive for β-gal staining was significantly increased in the Ang Ⅱ model group,and showed cell cycle arrest at G0/G1 phase with decreased S and G2/M phase ceil percentage,eNOS expression and cell viability（P〈0.05）.The extracts and telmisartan treatment of Ang II-induced cells resulted in decreased β-gal positive cells with a reduction in G0/G1phase cells and an increasing in S,G2/M phase ceils and eNOS expression（P〈0.05）.At 24 h,the extracts were more effective than telmisartan（P〈0.05）;while telmisartan was more effective at 48 h（P〈0.05）.Conclusion：Extracts from ginseng,notoginseng and chuanxiong can delay Ang II-induced aging of HUVECs and may play an important role in early senescence.

关 键 词：GINSENG notoginseng chuanxiong angiotensin Ⅱ vascular endothelial cells SENESCENCE 

分 类 号：R285[医药卫生—中药学]