Correlation between the different chain lengths of free fatty acid oxidation and abili .ty of trophoblastic invasion  

作　　者：Yu Huan Yang Zi Ding Xiaoyan Wang Yanling Han Yiwei 

机构地区：[1]Department of Obstetrics and Gynecology, Peking University ThirdHospital, Beijing 100191 [2]State Key Laboratory of Reproductive Biology, Institute of Zoology,Chinese Academy of Sciences, Beijing 1 00101, China

出　　处：《Chinese Medical Journal》2014年第19期3378-3382,共5页中华医学杂志（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （No. 81241021） and Beijing Municipal Natural Science Foundation （No. 7132215）.

摘　　要：Background Preeclampsia （PE） is associated with abnormal fatty acid beta-oxidation （FAO）, especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts. Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyI-CoA dehydrogenase （LCHAD） protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated. Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression （P 〈0.05） but no significant difference in CPT1 protein expression （P 〉0.05）. Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased （P 〈0.05）. Intracellular lipid droplets deposition was negatively correlated with invasivenss （R=-0.745, P 〈0.05）. Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.Background Preeclampsia （PE） is associated with abnormal fatty acid beta-oxidation （FAO）, especially metabolic disorders of long-chain fatty acid oxidation. The role of FAO dysfunction in inadequate invasion is unclear. The aim of this study was to explore the influence of various lengths fatty acids oxidation on invasiveness of trophoblasts. Methods Primary human trophoblast cells and HTR8/SVneo cells were treated with fatty acids of various lengths. Morphological changes, lipid deposition and ultrastructure changes of trophoblast cells were detected. Cells invasiveness was determined by transwell insert. CPT1, CPT2 and long-chain 3-hydroxyacyI-CoA dehydrogenase （LCHAD） protein expression were analyzed. The correlation between intracellular lipid droplets deposition and cells invasiveness was evaluated. Results Cells treated with long-chain fatty acids showed significant increased lipid droplets deposition, severe mitochondrial damage, decreased CPT2 and LCHAD protein expression （P 〈0.05） but no significant difference in CPT1 protein expression （P 〉0.05）. Invasiveness of the trophoblast cells of the LC-FFA group significantly decreased （P 〈0.05）. Intracellular lipid droplets deposition was negatively correlated with invasivenss （R=-0.745, P 〈0.05）. Conclusion Trophoblast cells after stimulation with long chain fatty acids exist fatty acid oxidation disorders, and reduce the ability of trophoblastic invasion.

关 键 词：fatty acid beta-oxidation long chain fatty acid TROPHOBLASTS INVASION 

分 类 号：R714.244[医药卫生—妇产科学]