Tim-3分子在胃癌患者外周血NK细胞上的表达及临床意义  

Expression and clinical significance of Tim- 3 on peripheral blood natural killer cells of gastric cancer patients

作　　者：顾海迪[1] 王彬[1] 王振欣[2] 朱金莲[2] 袁苏徐[2] 

机构地区：[1]南京医科大学附属苏州医院,苏州市立医院东区肿瘤内科,江苏苏州215001 [2]苏州大学附属第一医院肿瘤科,江苏苏州215006

出　　处：《现代肿瘤医学》2014年第10期2393-2395,共3页Journal of Modern Oncology

基　　金：江苏省普通高校研究生科研创新计划项目(编号:CXLX11_0084)

摘　　要：目的:检测Tim-3分子在胃癌荷瘤个体NK细胞上的表达,探讨Tim-3在胃癌发生发展中的作用。方法:收集2012年8月-2013年1月在苏州大学附属第一医院初治的原发性胃癌患者32例,健康体检者28例为正常对照组。流式细胞仪检测胃癌患者及正常对照组外周血NK细胞上Tim-3的表达,分析其与胃癌患者临床病理特征的关系。结果:胃癌患者外周血CD56+NK细胞上Tim-3的表达量为(49.27±21.06)%,显著高于健康对照组(37.00±17.16)%(P<0.05)。有淋巴结转移者Tim-3+CD56+NK细胞高表达率(55%)高于无淋巴结转移患者(0%);有远处转移患者Tim-3+CD56+NK细胞高表达率(100%)显著高于无远处转移者(40.7%),差异均有统计学意义(P<0.05)。Ⅲ-Ⅳ期胃癌患者Tim-3+CD56+NK细胞高表达率较Ⅰ-Ⅱ期患者高(73.7%vs 15.4%),差异具有显著性(P<0.01)。结论:Tim-3在胃癌的发生发展中发挥着一定作用,是潜在的用于评估胃癌预后的指标。Objective：To investigate the expression of Tim-3 on natural killer cells in tumor-bearing hosts with gastric cancer,and explore the role of Tim-3 in the development of gastric cancer. Methods：Thirty-two patients with newly diagnosed gastric cancer were involved in the study from August 2012 to January 2013,and 28 healthy vol-unteers were recruited as normal controls（ NC）. The expression of Tim-3 on NK cells in blood samples were evalua-ted with flow cytometry（ FCM）. Tim-3 expression on natural killer cells was correlated with clinicopathological pa-rameters. Results：Compared with normal controls,patients with gastric cancer showed higher proportion of Tim-3＋NK cells[（49. 27 ± 21. 06）% vs（37. 00 ± 17. 16）%,P〈0. 05]. The expression of Tim-3＋CD56＋NK cells in PBMC was much higher with the later clinical stage,positive lymph node and metastasis,gastric cancer patients with advanced disease（ stage Ⅲ -Ⅳ）were higher than those in early disease（ stage Ⅰ-Ⅱ）（73. 7% vs 15. 4%,P〈0.01）,and patients with positive lymph node（N1-3）were higher than those with negative（N0）（55% vs 0%,P〈0. 05）,patients with positive metastasis（ M1 ）were higher than those with negative（ M0 ）（100% vs 40. 7%,P〈0. 05）. Conclusion：Gastric cancer patients blood NK cells express higher levels of Tim-3 compared to healthy do-nors NK cells. Higher expression of Tim-3＋CD56＋NK cells in PBMC had positive correlation with the later clinical stage,positive lymph node and metastasis. Detecting the expression of Tim-3＋CD56＋NK cells would contribute to prediction of the disease progression and prognosis.

关 键 词：TIM-3 NK细胞 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]