Tspan 1及Integrin α6在人胰腺导管腺癌中的表达及其与临床特征的关系  

Expression and clinical significance of Tspan 1 and Integrin α6 in human pancreatic ductal adenocarcinoma

作　　者：石刚[1] 董明[1] 盛伟伟[1] 周建平[1] 于东洋[1] 孙巍[2] 

机构地区：[1]中国医科大学附属第一医院胃肠外科,沈阳110001 [2]沈阳市第四人民医院普通外科

出　　处：《中华外科杂志》2014年第10期781-786,共6页Chinese Journal of Surgery

基　　金：辽宁省科技厅科学事业公益研究基金（2013001008）;辽宁省教育厅特聘教授专项基金[辽财指教（2012）512]

摘　　要：目的 探讨Tspan 1及Integrinα6在胰腺导管腺癌（PDAC）及胰腺癌细胞中的表达与临床病理学参数的关系.方法 收集2004年5月至2013年1月收治的95例PDAC患者的组织石蜡切片及55例癌旁正常组织石蜡切片进行免疫组化检测.以Western blot法和实时定量（qRT）-PCR法检测16对配对冷冻保存的新鲜PDAC、癌旁组织及6株胰腺癌细胞中Tspan 1及Integrin α6蛋白和mRNA表达水平.分别采用x2检验、Spearman-rank相关分析、Kaplan-Meier法、多因素回归分析等对数据进行统计分析.结果 Tspan 1及Integrinα6在PDAC细胞膜表达高于癌旁胰腺组织（x2=7.429,P＜0.05;x2=15.100,p＜0.01）;Tspan 1表达与淋巴结转移、TNM分期及复发率呈正相关（x2=6.688,P＜0.01;x2=13.055,P＜0.01;x2 =6.116,P＜0.05）.Integrin α6表达与TNM分期呈正相关（x2=8.896,P＜0.05）;Tspan 1及Integrin α6的相关性有统计学意义（r=0.223,P＜0.05）;Tspan 1及Integrin α6高表达与生存期呈负相关（x2=5.263,P＜0.05;x2=10.124,P＜0.01）;多因素分析结果显示,Tspan 1及Integrinα6表达水平可以作为PDAC预后的独立预测因素（x2=6.152,P＜0.05;x2=9.479,P＜0.01）.16例胰腺癌组织中Tspan 1及Integrin α6的蛋白（t=2.278,P＜0.05;t=3.153,P＜0.05）和mRNA（t=2.439,P＜0.05;t=3.258,P＜0.05）表达均高于配对的癌旁正常组织;6株细胞株中Tspan 1及Integrin α6在蛋白水平及mRNA水平均有表达,来自转移灶的人胰腺腺癌细胞株SW1990中Tspan 1及Integrin α6在蛋白及mRNA水平表达高于来自原发灶的细胞株（P＜0.05）.结论 Tspan 1及Integrinα6表达对于PDAC的转移及浸润有促进作用,可能可以作为判断PDAC预后的独立因素.Objective To explore the clinicopathological significance and relationship of Tspan 1 and Integrin α6 expression in pancreatic ductal adenocarcinoma （PDAC） tissue and pancreatic cancer cell lines.Methods Immunohistochemistry was used to detect the expression of Tspan 1 and Integrin α6 in 95 paraffin-embedded PDAC specimens and 55 adjacent non-cancerous pancreatic tissues which were collected from May 2004 to January 2013.Western blot and quantitative real-time polymerase chain reaction （qRT-PCR） were used to detected the protein and mRNA expression in 16 paired fresh PDAC specimens of the pancreas and adjacent non-cancerous pancreatic tissues and 6 different pancreatic cancer cell lines.x test,Spearman-rank correlation analysis,Kaplan-Meier method and multivariate Cox regression analysis were used to analyze the data.Results Tspan 1 and Integrin α6 were significantly over-expressed in PDAC than in adjacent non-cancerous pancreatic tissues （x2=7.429,P 〈 0.05 ; x2 =15.1,P 〈 0.01）.Lymph node metastasis,TNM stage and post-operation recurrence were positively correlated with the expression of Tspan 1 （x2 =6.688,P 〈0.01 ;x2 =13.055,P 〈0.01 ;x2 =6.116,P 〈0.05）.TNM stage was positively correlated with the expression of Integrin α6 （x2 =8.896,P 〈 0.05）.Tspan 1 was correlated with Integrin α6 （r =0.223,P 〈0.05）.The expressions of Tspan 1 and Integrin α6 were negatively correlated with survival time （x2 =5.263,P 〈 0.05;x2 =10.124,P 〈 0.01）.Multivariate analysis revealed that Tspan 1 and Integrin α6 expressions were independent prognostic factors in PDAC patients （x2 =6.152,P 〈 0.05 ; x2 =9.479,P 〈 0.01）.Western blot（t=2.278,P〈0.05;t =3.153,P〈0.05）and qRT-PCR（t =2.439,P〈0.05;t =3.258,P 〈0.05） showed that Tspan 1 and Integrin α6 expressions were higher in PDAC tissues than in adjacent non-cancerous pancreatic.Tspan 1 and Integrin α6 were expressed in all six pancreatic cancer cell lines.In SW1990 which derived from metastasis PDAC,Tspan 1 and Integ

关 键 词：胰腺肿瘤 腺癌 Tspan 1 Integrinα6 四跨膜蛋白 

分 类 号：R735.9[医药卫生—肿瘤]