腺病毒介导的hTERTC27在体内外对小鼠胶质瘤的抑制作用  

Inhibitory effects of hTERTC27 mediated by adenoviral vector on mouse glioma carcinoma in vivo and in vitro

作　　者：龚汉贤[1] 李飞[1] 利玉欢[1] 刘清阁[1] 潘志信[1] 叶虹[1] 罗丽霞[1] 

机构地区：[1]南方医科大学附属南海人民医院神经内科,佛山528000

出　　处：《神经解剖学杂志》2014年第5期599-604,共6页Chinese Journal of Neuroanatomy

摘　　要：目的:探讨人端粒酶逆转录酶C-末端27kD多肽(hTERTC27)对小鼠胶质瘤的抑制作用及其可能的机制。方法:体外实验用PT-PCR法检测hTERTC27的cDNA水平,Western杂交鉴定hTERTC27蛋白的表达,hoechst法检测细胞的凋亡,CCK8法检测hTERTC27对GL261细胞的抑制率,及加入Caspsase-3的抑制剂ZdevdFMK后hTERTC27对GL261细胞抑制率的变化。体内实验建立小鼠胶质瘤模型,原位分别注射AdC27-EGFP、AdC27-EGFP+Zdevd-FMK和Ad-EGFP,观察小鼠的存活时间。结果:体外实验表明,hTERTC27能明显地抑制肿瘤细胞的生长,促进肿瘤的凋亡;而当加入Caspsase-3的抑制剂后,hTERTC27的抑制作用被明显阻断。体内实验显示,AdC27-EGFP小鼠组的生存时间为(40.38±11.30)d,高于AdC27-EGFP+Zdevd-FMK组的(27.25±6.41)d和Ad-EGFP组的(25.38±6.79)d(P<0.05)。结论:hTERTC27能在体内和体外明显地抑制小鼠胶质瘤的生长,其机制可能与Caspases-3凋亡途径有关。Objective： To investigate the inhibitory effects and the possible mechanism of human TERT C-terminal pep- tide （hTERTC27）on mice glioma carcinoma in vitro and in vivo. Methods： The expression of hTERTC27 cDNA was measured by reverse transcription polymerase chain reaction （RT-PCR）. The expression of hTERTC27 protein was exam- ined by Western blot. The apoptosis of ceils was investigated by hoechst assay. The variance of glioma cells＇ inhibition rate was measured by CCK8 assay after adding the Caspsase-3 inhibitor Zdevd-FMK. Then the mice models were estab- lished by injection of GL261 mice glioma cells into C57BL mice. The mice were treated by in situ injection of AdC27-EG- FP,AdC27-EGFP ＋ Zdevd-FMK and Ad-EGFP. The survival time of remaining tumor-bearing mice were observed. Re- suit： hTERTC27 inhibited the growth of GL261 mice glioma cells in vitro, the inhibition was intercepted by adding the Caspsase-3 inhibitor. The survival day of mice was （40.38±11.30）days in Ad-hTERTC27 group, （27.25±6.41 ）days in AdC27-EGFP ＋ Zdevd-FMK group and（ 25.38±6.79 ）days in Ad-EGFP groups. The differences among these three groups were significant （ P 〈 0.05 ）. Conclusion ： hTERTC27 could effectually inhibit the growth of mice glioma carcino- ma in vitro and in vivo, and its mechanism was possibly related with Caspsase-3 apoptosis pathway.

关 键 词：hTERTC27 Caspases-3 胶质瘤 基因治疗 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤]